Determination of ritonavir, a new HIV protease inhibitor, in biological samples using reversed-phase high-performance liquid chromatography

被引:71
作者
Marsh, KC [1 ]
Eiden, E [1 ]
McDonald, E [1 ]
机构
[1] Abbott Labs, Div Pharmaceut Prod, Drug Anal Dept, Abbott Pk, IL 60064 USA
来源
JOURNAL OF CHROMATOGRAPHY B | 1997年 / 704卷 / 1-2期
关键词
ritonavir;
D O I
10.1016/S0378-4347(97)00454-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple, accurate and precise high-performance Liquid chromatographic method has been developed for measurement of ritonavir concentrations in human plasma. Ritonavir was partitioned from the plasma using liquid-liquid extraction with a mixture of ethyl acetate and hexane at neutral pH, with an average recovery >80%. Following two sequential washings of the reconstituted sample with hexane, chromatographic separation was accomplished on a C-18 analytical column with a mobile phase containing acetonitrile, methanol and 0.01 M tetramethylammonium perchlorate in 0.1% aqueous trifluoroacetic acid (40:5:55, v/v) with low wavelength UV detection at 205 nm. Standard curves were linear (r(2)>0.9998) over the concentration range 0.01-15 mu g/ml with both inter- and intra-day coefficients of variation typically less than 5%. The stability of ritonavir in plasma was excellent, with no evidence of degradation after 5 days at room temperature or after 6 months in a freezer. Decontamination procedures for HIV-positive plasma samples showed 5.6 and 10.2% degradation following heating to 60 degrees C for 30 or 60 min, respectively. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:307 / 313
页数:7
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