Biocompatible Platinum Nanoclusters Prepared Using Bitter Gourd Polysaccharide for Colorimetric Detection of Ascorbic Acid

被引：14

作者：

Liu, Kai ^{[1
]}

Zhao, Yu ^{[1
]}

Zhang, Lu ^{[1
]}

He, Mengmeng ^{[1
]}

Lin, Weifeng ^{[2
]}

Sun, Haotian ^{[3
]}

Liu, Zhiwei ^{[1
]}

Hu, Jie ^{[1
]}

Wang, Longgang ^{[1
]}

机构：

[1] Yanshan Univ, State Key Lab Metastable Mat Sci & Technol, Key Lab Appl Chem, Hebei Key Lab Heavy Met Deep Remediat Water & Res, Qinhuangdao 066004, Hebei, Peoples R China

[2] Weizmann Inst Sci, Dept Mol Chem & Mat Sci, IL-76100 Rehovot, Israel

[3] Ocean NanoTech LLC, San Diego, CA 92126 USA

来源：

BIOMOLECULES | 2021年 / 11卷 / 05期

基金：

中国博士后科学基金;

关键词：

green synthesis; colorimetric; bitter gourd polysaccharide; ascorbic acid; detection; MOMORDICA-CHARANTIA L; NANOPARTICLES; ANTIOXIDANT;

D O I：

10.3390/biom11050647

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ascorbic acid is an organic compound with antioxidant properties that can protect the human body from the threat of free radicals. Therefore, it is important to detect the existence and measure the concentration of ascorbic acid to regulate its content in the human body. In this work, we prepared bitter gourd polysaccharide (BGP)-stabilized platinum nanoclusters (Pt-BGP NCs) by reacting BGP with K2PtCl4. Pt-BGP NCs and catalyzed the decomposition of H2O2 to generate center dot OH radicals, which could oxidize TMB to form oxidized TMB (oxTMB), indicating their peroxidase-like properties. The kinetics followed the Michaelis-Menten equation. Furthermore, the colorimetric detection of ascorbic acid using Pt-BGP NCs showed high selectivity and a low detection limit of 0.191 mu M. The accuracy of real sample detection using Pt-BGP NCs was as high as 98.9%. More importantly, Pt-BGP NCs had high cell biocompatibility and extremely low hemolysis rate due to the component of BGP. In summary, the prepared Pt-BGP NCs with reductive activity and good biocompatibility have good application prospects in colorimetric detection of ascorbic acid.

引用

页数：12

共 32 条

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