Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

被引：18

作者：

Gonzalez-Vera, Juan A. ^{[1
]}

Bouzada, David ^{[2
,3
]}

Bouclier, Celine ^{[1
]}

Eugenio Vazquez, M. ^{[2
,3
]}

Morris, May C. ^{[1
]}

机构：

[1] Univ Montpellier, CNRS, IBMM UMR 5247, Inst Biomol Max Mousseron, 15 Av Charles Flahault, F-34093 Montpellier, France

[2] Univ Santiago de Compostela, Ctr Singular Invest Quim Biol & Mat Mol CIQUS, Santiago De Compostela 15782, Spain

[3] Univ Santiago de Compostela, Dept Quim Organ, Santiago De Compostela 15782, Spain

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 45期

关键词：

THERAPEUTIC TARGET; PHOSPHORYLATION; MELANOMA; PROBES; CDK4; VERSATILE; COMPLEX; ASSAY; TIME;

D O I：

10.1039/c6cc09948c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety.

引用

收藏

页码：6109 / 6112

页数：4

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