Main and epistatic loci studies in soybean for Sclerotinia sclerotiorum resistance reveal multiple modes of resistance in multi-environments

被引：59

作者：

Moellers, Tara C. ^{[1
]}

Singh, Arti ^{[1
]}

Zhang, Jiaoping ^{[1
]}

Brungardt, Jae ^{[1
]}

Kabbage, Mehdi ^{[2
]}

Mueller, Daren S. ^{[3
]}

Grau, Craig R. ^{[2
]}

Ranjan, Ashish ^{[2
]}

Smith, Damon L. ^{[2
]}

Chowda-Reddy, R. V. ^{[1
]}

Singh, Asheesh K. ^{[1
]}

机构：

[1] Iowa State Univ, Dept Agron, Ames, IA 50011 USA

[2] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA

[3] Iowa State Univ, Dept Plant Pathol, Ames, IA 50011 USA

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

GENOME-WIDE ASSOCIATION; VACUOLAR GLUCOSE EXPORTER; SUDDEN-DEATH SYNDROME; GLYCINE-MAX; STEM ROT; SALICYLIC-ACID; PLANT INTRODUCTIONS; GENETIC DIVERSITY; AGRONOMIC TRAITS; ARABIDOPSIS;

D O I：

10.1038/s41598-017-03695-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genome-wide association (GWAS) and epistatic (GWES) studies along with expression studies in soybean [Glycine max (L.) Merr.] were leveraged to dissect the genetics of Sclerotinia stem rot (SSR) [caused by Sclerotinia sclerotiorum (Lib.) de Bary], a significant fungal disease causing yield and quality losses. A large association panel of 466 diverse plant introduction accessions were phenotyped in multiple field and controlled environments to: (1) discover sources of resistance, (2) identify SNPs associated with resistance, and (3) determine putative candidate genes to elucidate the mode of resistance. We report 58 significant main effect loci and 24 significant epistatic interactions associated with SSR resistance, with candidate genes involved in a wide range of processes including cell wall structure, hormone signaling, and sugar allocation related to plant immunity, revealing the complex nature of SSR resistance. Putative candidate genes [for example, PHYTOALEXIN DEFFICIENT 4 (PAD4), ETHYLENE-INSENSITIVE 3-LIKE 1 (EIL3), and ETHYLENE RESPONSE FACTOR 1 (ERF1)] clustered into salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) pathways suggest the involvement of a complex hormonal network typically activated by both necrotrophic (ET/JA) and biotrophic (SA) pathogens supporting that S. sclerotiorum is a hemibiotrophic plant pathogen.

引用

页数：13

共 86 条

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