A miR-212-3p/SLC6A1 Regulatory Sub-Network for the Prognosis of Hepatocellular Carcinoma

被引：8

作者：

Zhang, Dan-Dan ^{[1
]}

Wang, Wen-Er ^{[2
]}

Ma, Yu-Shui ^{[3
]}

Shi, Yi ^{[3
]}

Yin, Jie ^{[4
]}

Liu, Ji-Bin ^{[5
]}

Yang, Xiao-Li ^{[6
]}

Xin, Rui ^{[6
]}

Fu, Da ^{[6
]}

Zhang, Wen Jie ^{[1
,7
]}

机构：

[1] Shihezi Univ, Dept Pathol, Sch Med, Shibei 2nd Rd, Shihezi 832002, Xinjiang, Peoples R China

[2] Peoples Hosp Xiangxi Autonomous Prefecture, Dept Hepatobiliary Surg, Jishou 416000, Hunan, Peoples R China

[3] Second Mil Med Univ, Natl Ctr Liver Canc, Eastern Hepatobiliary Surg Hosp Inst, Int Cooperat Lab Signal Transduct, Shanghai 200433, Peoples R China

[4] Haian Peoples Hosp, Dept Gen Surg, Haian 226600, Jiangsu, Peoples R China

[5] Nantong Tumor Hosp, Canc Inst, Nantong 226631, Peoples R China

[6] Tongji Univ, Shanghai Peoples Hosp 10, Cent Lab Med Res, Sch Med, 36 Yunxin Rd, Shanghai 200072, Peoples R China

[7] Shihezi Univ, Sch Med, Key Labs Xinjiang Endem & Ethn Dis, Shihezi 832002, Xinjiang, Peoples R China

来源：

CANCER MANAGEMENT AND RESEARCH | 2021年 / 13卷

基金：

中国国家自然科学基金;

关键词：

HCC; ceRNA; Myc; SLC6A1; prognosis; HCC CELLS; LIVER; CANCER; EXPRESSION; IDENTIFICATION; PROLIFERATION; METABOLISM; APOPTOSIS; MIGRATION; INVASION;

D O I：

10.2147/CMAR.S308986

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Introduction: Hepatocellular carcinoma (HCC) is a liver cancer with a poor prognosis. Owing to the complexity and limited pathogenic mechanism research on HCC, the molecular targeted therapy has been hindered. Methods: In this study, we categorized transcriptome data into low-Myc and high-Myc expression groups in 365 HCC samples, screened the differentially expressed RNAs, including 441 DE-lncRNAs, 99 DE-miRNAs and 612 DE-mRNAs, constructed a lncRNA-miRNA -mRNA regulatory network, and selected a hub triple regulatory network through cytoHubba analysis. Through Gene ontology and KEGG pathway, a hub regulatory network was particularly enriched in the "Wnt signaling pathway" and "Cytochrome P450-arranged by substrate type" by Metascape. The prognostic genes in the hub regulatory network were evaluated by the RNA expression analysis, Kaplan-Meier (KM) survival analysis, and correlation analysis. Results: The results showed that miR-212-3p/SLC6A1 axis was a potential prognostic model for HCC. Furthermore, IHC analysis showed down-regulated expression of SLC6A1 in HCC tissues and Alb-Cre;Myc mouse liver cancer tissues. The genetics and epigenetic analysis indicated that SLC6A1 expression was negatively correlated with DNA methylation. Immune infiltration analysis showed a negative relation between SLC6A1 and T cell exhaustion/monocyte in liver cancer tissues. Conclusion: In summary, the study revealed that miR-212-3p/SLC6A1 axis could serve as a crucial therapeutic target for HCC.

引用

页码：5063 / 5075

页数：13

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