Development of a monoclonal antibody-based immunochromatographic strip for cephalexin

被引：62

作者：

Guo, Jingnan ^{[1
]}

Liu, Liqiang ^{[1
]}

Xue, Feng ^{[2
]}

Xing, Changrui ^{[1
]}

Song, Shanshan ^{[1
]}

Kuang, Hua ^{[1
]}

Xu, Chuanlai ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi, Peoples R China

[2] Jiangsu Entry & Exit Inspect & Quarantine Bur, Nanjing, Jiangsu, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2015年 / 26卷 / 02期

关键词：

test strip; monoclonal antibody; milk; cephalexin; TANDEM MASS-SPECTROMETRY; BETA-LACTAM ANTIBIOTICS; LIQUID-CHROMATOGRAPHY; RAW-MILK; RESIDUE; ASSAY; VALIDATION; ELISA; FOOD; IMMUNOASSAY;

D O I：

10.1080/09540105.2014.907242

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An anti-cephalexin (CEX) monoclonal antibody was prepared by the immunogen of CEX-keyhole limpet hemocyanin connected by disuccinimidyl suberate, which was used for the establishment of a rapid immunochromatographic test strip. With this semi-quantitative detection method, a low limit of deduction of 10 ng mL(-1) was obtained with the naked eyes and 1.3 +/- 0.1 ng mL(-1) by a strip reader in unprocessed milk within 5 min, which was much lower than the European Union Maximum Residue Limit of 100 mu g kg(-1) in milk. The recovery in the range of 87-120% was measured in another milk sample. In conclusion, this method with high sensitivity and satisfactory recovery shows significant potential for CEX residue detection in milk.

引用

页码：282 / 292

页数：11

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