Toward a standardization of the microbial inoculum for ready biodegradability testing of chemicals

被引:10
作者
Vazquez-Rodriguez, G [1 ]
Goma, G [1 ]
Rols, JL [1 ]
机构
[1] Inst Natl Sci Appl, UMR CNRS 5504, UR INRA 792, Ctr Bioingn Gilbert Durand, F-31077 Toulouse 4, France
关键词
activated sludge; inoculum; normalized tests; ready biodegradability; xenobiotics;
D O I
10.2166/wst.2000.0493
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Activated sludge constitutes a widely used inoculum source in biodegradability tests, due principally to its microbial diversity and ubiquity. In ready biodegradability tests, the composition and the quantity of this type of inoculum are the major factors which affect the reproducibility of results. Experiments with dedecylbenzene sulfonate were carried out to evaluate the impact of the non-controlled inoculum on test response. At low inoculation levels, cellular growth and soluble residual carbon accumulation were favoured and low mineralization levels were obtained. A procedure for inoculum preparation was proposed it consists in a pre-treatment of activated sludge, collected in different municipal wastewater treatment plants, by centrifugation (500 g for 4 minutes) which enabled the supernatant cellular density (CFU concentration) to be more uniform than with the conventional decantation procedure (30 min).
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页码:43 / 46
页数:4
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