Serum miRNAs miR-206, 143-3p and 374b-5p as potential biomarkers for amyotrophic lateral sclerosis (ALS)

被引：111

作者：

Waller, Rachel ^{[1
]}

Goodall, Emily F. ^{[1
]}

Milo, Marta ^{[2
]}

Cooper-Knock, Jonathan ^{[1
]}

Da Costa, Marc ^{[1
]}

Hobson, Esther ^{[1
]}

Kazoka, Mbombe ^{[1
]}

Wollff, Helen ^{[1
]}

Heath, Paul R. ^{[1
]}

Shaw, Pamela J. ^{[1
]}

Kirby, Janine ^{[1
]}

机构：

[1] Univ Sheffield, Sheffield Inst Translat Neurosci, Dept Neurosci, Sheffield, S Yorkshire, England

[2] Univ Sheffield, Dept Biomed Sci, Sheffield, S Yorkshire, England

来源：

NEUROBIOLOGY OF AGING | 2017年 / 55卷

基金：

英国医学研究理事会; 瑞典研究理事会; 美国国家卫生研究院;

关键词：

Amyotrophic lateral sclerosis; Serum; MicroRNA; Polymerase chain reaction; Biomarker; CIRCULATING MICRORNAS; HEXANUCLEOTIDE REPEAT; PCR DATA; DIAGNOSIS; BLOOD; EXPRESSION; CANCER; C9ORF72; GENES; DELAY;

D O I：

10.1016/j.neurobiolaging.2017.03.027

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Amyotrophic lateral sclerosis (ALS) is a fatal, neurodegenerative condition characterized by loss of motor neurones and progressive muscle wasting. There is no diagnostic test for ALS therefore robust biomarkers would not only be valuable for diagnosis, but also for the classification of disease subtypes, monitoring responses to drugs and tracking disease progression. As regulators of gene expression, microRNAs (miRNAs) are increasingly used for diagnostic and prognostic purposes in various disease states with increasing exploration in neurodegenerative disorders. We hypothesize that circulating blood-based miRNAs will serve as biomarkers and use miRNA profiling to determine miRNA signatures from the serum of sporadic ALS patients compared to healthy controls and patients with diseases that mimic ALS. A number of differentially expressed miRNAs were identified in each set of patient comparisons. Validation in an additional patient cohort showed that miR-206 and miR-143-3p were increased and miR-374b-5p was decreased compared to controls. A continued change in miRNA expression persisted during disease progression indicating the potential use of these particular miRNAs as longitudinal biomarkers in ALS. (C) 2017 The Authors. Published by Elsevier Inc.

引用

页码：123 / 131

页数：9

共 43 条

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