Colony immunoblot assay for the detection of hemolysin BL enterotoxin producing Bacillus cereus

被引：23

作者：

Moravek, M ^{[1
]}

Wegscheider, M ^{[1
]}

Schulz, A ^{[1
]}

Dietrich, R ^{[1
]}

Bürk, C ^{[1
]}

Märtlbauer, E ^{[1
]}

机构：

[1] Univ Munich, Fac Vet, Inst Hyg & Technol Food Anim Origin, D-85764 Munich, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2004年 / 238卷 / 01期

关键词：

Bacillus cereus; colony immunoblot; enterotoxin; hemolysin BL; non-hemolytic enterotoxin;

D O I：

10.1016/j.femsle.2004.07.024

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. To facilitate the identification of hemolysin BL-enterotoxin complex (HBL) and/or the nonhemolytic enterotoxin (NHE) producing colonies a colony immunoblot procedure was developed, which allows a fast and easy identification of the respective colonies from blood agar plates. The enterotoxins were transferred from the blood agar medium to a nitrocellulose membrane and the immobilized toxins were probed with monoclonal antibodies. The antibodies 2A3 and 1A8 allowed the specific detection of the B component of HBL and the nheA component Of NHE. The assay enabled the reliable identification of HBL expressing colonies and differentiation from NHE producing but HBL negative colonies. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：107 / 113

页数：7

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