Amazon Fruits Inhibit Growth and Promote Pro-apoptotic Effects on Human Ovarian Carcinoma Cell Lines

被引：16

作者：

de Souza, Vanessa Rosse ^{[1
]}

Menezes Brum, Mariana Concentino ^{[2
]}

Guimaraes, Isabella dos Santos ^{[3
]}

dos Santos, Paula de Freitas ^{[2
]}

do Amaral, Thuane Oliveira ^{[1
]}

Abreu, Joel Pimentel ^{[1
]}

Passos, Thuane ^{[1
]}

Freitas-Silva, Otniel ^{[4
]}

Pereira Gimba, Etel Rodrigues ^{[2
,5
]}

Teodoro, Anderson Junger ^{[1
]}

机构：

[1] Univ Fed Estado Rio de Janeiro, Lab Funct Foods, BR-22290240 Rio De Janeiro, Brazil

[2] Inst Nacl Canc, Res Ctr, Cellular & Mol Oncobiol Program, BR-20231050 Rio De Janeiro, Brazil

[3] Inst Nacl Canc, Res Ctr, Div Clin Res, BR-20231050 Rio De Janeiro, Brazil

[4] Empresa Brasileira Pesquisa Agr, Embrapa Agroind Alimentos, BR-23020470 Rio De Janeiro, Brazil

[5] Univ Fed Fluminense, Dept Nat Sci, BR-28895532 Rio Das Ostras, Brazil

来源：

BIOMOLECULES | 2019年 / 9卷 / 11期

关键词：

Byrsonima crassifolia; Byrsonima verbascifolia; Spondias mombin; carotenoids; cisplatin; ovarian cancer; REACTIVE OXYGEN; CANCER; CAROTENOIDS; QUANTIFICATION; IDENTIFICATION; PROLIFERATION;

D O I：

10.3390/biom9110707

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Murici (Byrsonima crassifolia (L.) Kunth and B. verbascifolia (L.) DC.) and tapereba (Spondias mombin) are Amazonian fruits that contain bioactive compounds. Biochemical and molecular characterization of these fruits can reveal their potential use in preventing diseases, including cancer. The extracts were characterized regarding the presence and profile of carotenoids by high-performance liquid chromatography (HPLC), total phenolic content by the Folin-Ciocalteu assay, and antioxidant activity by antioxidant value 2,2-diphenyl-1-picrylhydrazyl (DPPH) content analysis, 22,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) content analysis, Ferric-Reducing Ability of Plasma (FRAP), and Oxygen Radical Absorbance Capacity (ORAC) analysis. The extracts of tapereba and murici studied were important sources of total carotenoids and lutein, respectively. The extracts were then tested for their effect on the viability of the A2780 ovarian cancer (OC) cell line and its cisplatin (CDDP)-resistant derived cell line, called ACRP, by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. Their influence on cell cycle and apoptosis were analyzed by using flow cytometry. Murici and tapereba cell extracts exhibited a strong bioactivity by inhibiting A2780 and ACRP cell viability by 76.37% and 78.37%, respectively, besides modulating the cell cycle and inducing apoptotic cell death. Our results open new perspectives for the development of innovative therapeutic strategies using these Amazon fruit extracts to sensitize ovarian cancer cells to current chemotherapeutic options.

引用

页数：15