Cytokeratin 18 expression pattern correlates with renal cell carcinoma progression: Relationship with Snail

被引：21

作者：

Messai, Yosra ^{[1
,2
]}

Noman, Muhammad Zaeem ^{[1
]}

Derouiche, Amine ^{[3
]}

Kourda, Nadia ^{[4
]}

Akalay, Intissar ^{[1
]}

Hasmim, Meriem ^{[1
]}

Stasik, Izabela ^{[1
]}

Ben Jilani, Sarra ^{[2
]}

Chebil, Mohamed ^{[3
]}

Caignard, Anne ^{[5
]}

Azzarone, Bruno ^{[6
]}

Gati, Asma ^{[2
]}

Elgaaied, Amel Ben Ammar ^{[2
]}

Chouaib, Salem ^{[1
]}

机构：

[1] Inst Gustave Roussy, INSERM, Lab Immunol Tumeurs Humaines Interact Effecteuis, U753, F-94805 Villejuif, France

[2] Fac Sci Tunis, Lab Genet Immunol & Pathol Humaines, Tunis, Tunisia

[3] Hop Charles Nicolle Tunis, Serv Urol, Tunis, Tunisia

[4] Hop Charles Nicolle Tunis, Lab Cytol & Immunopathol, Tunis, Tunisia

[5] Univ Paris 05, INSERM, CNRS, Inst Cochin,UMR 8104,U567, Paris, France

[6] Univ Paris 11, Hop Paul Brousse, INSERM, UMR 542, Villejuif, France

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2010年 / 36卷 / 05期

关键词：

renal cell carcinoma; CK18; tumor progression; Snail; MONOCLONAL-ANTIBODIES; TUMOR SUSCEPTIBILITY; BREAST CARCINOMAS; T-CELLS; CANCER; OVEREXPRESSION; MANAGEMENT; INDUCTION; INDICATOR; ANTIGENS;

D O I：

10.3892/ijo_00000597

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Renal cell carcinoma (RCC) is the most common type of kidney cancer and recent developments in the molecular biology of RCC have identified multiple pathways associated with the development of this cancer. This study aimed at analyzing the expression pattern of cytokeratin 18 (CK18) in RCC patients and its prognostic relevance. We quantified CK18 mRNA expression and protein using real-time reverse transcription quantitative polymcrase chain reaction (RT-QPCR) and immunohistochemistry. respectively, in paired tumor and non-tumor samples from 42 patients. Our data indicate that CK18 mRNA and proteins levels increased with advanced stage and grade of the disease. Using primary (RCC5) and metastatic renal cell carcinoma (RCC5 met) cell lines, we demonstrated that CK18 expression was 5-fold higher in the metastatic as compared to the primary RCC cell line and correlated with a migratory phenotype characterized by a distinct elongated morphology as revealed by Phalloidin staining In addition, RCC5 met cells displayed an increased capacity to attach to fibronectin and collagen which was lost following CK18 knock-down. Our data also indicate that the expression of CK18 was associated with increased Snail expression which correlated positively with advanced disease in RCC patients The present Findings suggest that CK18 may play an important role in the progression of RCC and it may be used as a new predictor for RCC

引用

页码：1145 / 1154

页数：10

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