Measuring protein-protein interactions

被引：73

作者：

Lakey, JH ^{[1
]}

Raggett, EM ^{[1
]}

机构：

[1] Univ Newcastle Upon Tyne, Sch Med, Dept Biochem & Genet, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1998年 / 8卷 / 01期

关键词：

D O I：

10.1016/S0959-440X(98)80019-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The binding of one protein to another provokes a variety of biophysical changes that can then be used as a measure of the binding reaction. Optical spectroscopy, particularly fluorescence, is the most flexible technique, but surface plasmon resonance biosensors, microcalorimetry and mass spectroscopy have recently shown significant development.

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收藏

页码：119 / 123

页数：5

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共 56 条

[1] Individual rate constants for the interaction of Ras proteins with GTPase-activating proteins determined by fluorescence spectroscopy [J].

Ahmadian, MR ;

Hoffmann, U ;

Goody, RS ;

Wittinghofer, A .

BIOCHEMISTRY, 1997, 36 (15) :4535-4541

[2] Structure-function analysis of TAF130: Identification and characterization of a high-affinity TATA binding protein interaction domain in the N terminus of yeast TAF(II)130 [J].

Bai, Y ;

Perez, GM ;

Beechem, JM ;

Weil, PA .

MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (06) :3081-3093

[3] Evaluation of linked protonation effects in protein binding reactions using isothermal titration calorimetry [J].

Baker, BM ;

Murphy, KP .

BIOPHYSICAL JOURNAL, 1996, 71 (04) :2049-2055

[4] Dissecting the energetics of a protein-protein interaction: The binding of ovomucoid third domain to elastase [J].

Baker, BM ;

Murphy, KP .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 268 (02) :557-569

[5] Imaging the intracellular trafficking and state of the AB(5) quaternary structure of cholera toxin [J].

Bastiaens, PIH ;

Majoul, IV ;

Verveer, PJ ;

Soling, HD ;

Jovin, TM .

EMBO JOURNAL, 1996, 15 (16) :4246-4253

[6] Analysis of interacting biopolymer systems by analytical ultracentrifugation [J].

Behlke, J ;

Ristau, O .

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 1997, 25 (5-6) :325-332

[7] BOUND WATER-MOLECULES AND CONFORMATIONAL STABILIZATION HELP MEDIATE AN ANTIGEN-ANTIBODY ASSOCIATION [J].

BHAT, TN ;

BENTLEY, GA ;

BOULOT, G ;

GREENE, MI ;

TELLO, D ;

DALLACQUA, W ;

SOUCHON, H ;

SCHWARZ, FP ;

MARIUZZA, RA ;

POLJAK, RJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (03) :1089-1093

[8] New developments in analytical ultracentrifugation and related macromolecular modelling techniques [J].

Byron, O ;

Harding, SE .

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 1997, 25 (5-6) :305-306

[9] Peptide ligands of pp60(c-src) SH2 domains: A thermodynamic and structural study [J].

Charifson, PS ;

Shewchuk, LM ;

Rocque, W ;

Hummel, CW ;

Jordan, SR ;

Mohr, C ;

Pacofsky, GJ ;

Peel, MR ;

Rodriguez, M ;

Sternbach, DD ;

Consler, TG .

BIOCHEMISTRY, 1997, 36 (21) :6283-6293

[10] A GENERAL-METHOD TO RECONDITION AND REUSE BIACORE SENSOR CHIPS FOULED WITH COVALENTLY IMMOBILIZED PROTEIN PEPTIDE [J].

CHATELIER, RC ;

GENGENBACH, TR ;

GRIESSER, HJ ;

BRIGHAMBURKE, M ;

OSHANNESSY, DJ .

ANALYTICAL BIOCHEMISTRY, 1995, 229 (01) :112-118

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