A fish specific expression vector containing the interferon regulatory factor 1A (IRF1A) promoter for genetic immunization of fish

被引：17
作者：
Alonso, M
Johnson, M
Simon, B
Leong, JA
机构：
[1] Oregon State Univ, Dept Microbiol, Ctr Salmon Dis Res, Corvallis, OR 97330 USA
[2] Cornell Univ, Dept Biochem, Ithaca, NY 14853 USA
[3] Vet Adm Med Ctr, Portland, OR 97201 USA
[4] Univ Hawaii, Hawaii Inst Marine Biol, Kaneohe, HI 96744 USA
来源：
VACCINE | 2003年 / 21卷 / 15期
关键词：
DNA vaccines; IHNV; interferon regulatory factor 1a (IRF1A); promoters;
D O I：
10.1016/S0264-410X(02)00749-1
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
DNA plasmid vectors were constructed with inducible rainbow trout promoters to take the place of the cytomegalovirus immediate early promoter in DNA vaccines for fish. DNA fragments containing the promoter regions upstream of the rainbow trout Mx1 and interferon regulatory factor 1A (IRF1A) genes were obtained and their nucleotide sequences were determined. Vectors containing the G gene of IHNV linked to the trout promoters were found to be very effective as vaccines in rainbow trout. In addition, we demonstrated that the IRF1A promoter is transcriptionally active in mouse cells NIH-3T3 suggesting that this fish promoter might be used for mammalian DNA vaccine development as well. (C) 2002 Published by Elsevier Science Ltd.
引用
收藏
页码：1591 / 1600
页数：10
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