Catalytic activity of lipid-coated enzymes in organic media

被引：3

作者：

Okahata, Y ^{[1
]}

Mori, T ^{[1
]}

机构：

[1] Tokyo Inst Technol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 226, Japan

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 1997年 / 73卷 / 10期

关键词：

lipid-coated enzyme; enzyme reaction in organic solvents; lipase; phospholipase D; beta-D-galactosidase;

D O I：

10.2183/pjab.73.210

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A new way of solubilizing hydrolytic enzymes in organic media was achieved by coating the enzyme with lipid monolayers. Lipid-coated enzymes have been used successfully for a number of types of reactions in organic media or organic-aqueous two-phases, for example enantioselective esterification by lipase, transphosphatidylation of water-insoluble phospholipids by phospholipase D, hydrolysis of a lipophilic substrate by a catalytic antibody, and transglycosylation by beta-D-galactosidase.

引用

页码：210 / 214

页数：5

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