Kinetics of c-phycocyanin reaction with hypochlorite

被引:21
作者
Romay, C
Gonzalez, R
Pizarro, M
Lissi, E [1 ]
机构
[1] Univ Santiago Chile, Fac Quim & Biol, Dept Quim, Santiago, Chile
[2] CNIC, Natl Ctr Sci Res, Dept Pharmacol, Havana, Cuba
来源
JOURNAL OF PROTEIN CHEMISTRY | 2000年 / 19卷 / 02期
关键词
c-phycocyanin; hypopchlorite; bilin modification; reaction rate;
D O I
10.1023/A:1007038801482
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hydrochlorous acid bleaches c-phycocyanin visible absorbance with a second-order rate constant (pH 7.4) of 1.3 x 10(3) M-1 s(-1). In excess of protein, ca. 0.16 bilin moieties are disrupted by each reacted HOCl molecule. This indicates that the main reaction takes place at the apoprotein level, with a total rate constant (in monomeric units concentration) of 2.5 x 10(4) M-1 s(-1). This rate constant is too low to provide protection to other biomolecules under physiological conditions. The reported antiinflammatory properties of phycocyanin are not then related to the removal of HOCl. On the other hand, the rather slow reaction rate with HOCl could be beneficial to its role as antiinflammatory agent since it will allow the protein to maintain its integrity at the inflammation locus.
引用
收藏
页码:151 / 155
页数:5
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