Dietary α-linolenic acid increases TNF-α, and decreases IL-6, IL-10 in response to LPS:: effects of sesamin on the Δ-5 desaturation of ω6 and ω3 fatty acids in mice

被引:58
作者
Chavali, SR
Zhong, WW
Forse, RA
机构
[1] Beth Israel Deaconess Med Ctr, Harvard Inst Med, Dept Surg, Surg Metab Lab, Boston, MA 02215 USA
[2] Harvard Univ, Sch Med, Boston, MA 02215 USA
来源
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1998年 / 58卷 / 03期
关键词
D O I
10.1016/S0952-3278(98)90112-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sesamin (a non-fat portion of sesame seed oil) inhibits Delta-5 desaturase activity resulting in an accumulation of dihomo-gamma-linolenic acid (DGLA) which can displace arachidonic acid (AA) and decrease the formation of pro-inflammatory mediators. We investigated the effects of consumption of diets containing 0.25wt% sesamin and 15 wt% safflower oil (SO) (providing 12% of the added fat as linoleic acid) or a 15 wt% 2:1 mixture of linseed oil and SO (LOSO) (providing 6% alpha-linolenic acid and 6% linoleic acid) for 3 weeks on the liver membrane fatty acid composition and on the production of prostaglandin (PG) E-2, TNF-alpha, IL-6 and IL10 in mice. Consumption of sesamin-supplemented SO and LOSO diets resulted in a significant increase in the levels of 20:3 omega 6 (DGLA), suggesting that sesamin inhibited Delta-5 desaturation of omega 6 fatty acids. In animals fed LOSO diets, the levels of alpha-linolenic acid, eicosapentaenoic acid (EPA) and of docosahexaenoic acid (DHA) were elevated with a concomitant decrease of arachidonic acid (AA) in the liver membrane phospholipids. Further, in animals fed LOSO diets with or without sesamin, an increase in the circulating levels of TNF-alpha was associated with a concomitant decrease in PGE(2). Despite a lack of differences in the levels of AA, the PGE(2) levels were significantly lower in mice fed sesamin-supplemented SO compared to those fed SO alone. Thus, these data suggest that irrespective of the availability of a specific fatty acid as a substrate, through regulating the PGE(2) synthesis, the production of TNF-alpha could be modulated.
引用
收藏
页码:185 / 191
页数:7
相关论文
共 41 条
[1]   PROTECTIVE EFFECTS OF SESAMIN AGAINST LIVER-DAMAGE CAUSED BY ALCOHOL OR CARBON-TETRACHLORIDE IN RODENTS [J].
AKIMOTO, K ;
KITAGAWA, Y ;
AKAMATSU, T ;
HIROSE, N ;
SUGANO, M ;
SHIMIZU, S ;
YAMADA, H .
ANNALS OF NUTRITION AND METABOLISM, 1993, 37 (04) :218-&
[2]  
ALTAVILLA D, 1966, PHARMACEUT RES, V33, P203
[3]  
APPELBERG R, 1994, IMMUNOLOGY, V82, P361
[4]   INTERLEUKIN-10 IS A CENTRAL REGULATOR OF THE RESPONSE TO LPS IN MURINE MODELS OF ENDOTOXIC-SHOCK AND THE SHWARTZMAN REACTION BUT NOT ENDOTOXIN TOLERANCE [J].
BERG, DJ ;
KUHN, R ;
RAJEWSKY, K ;
MULLER, W ;
MENON, S ;
DAVIDSON, N ;
GRUNIG, G ;
RENNICK, D .
JOURNAL OF CLINICAL INVESTIGATION, 1995, 96 (05) :2339-2347
[5]   Immune complex-induced interleukin-6, interleukin-10 and prostaglandin secretion by human monocytes: A network of pro- and anti-inflammatory cytokines dependent on the antigen:antibody ratio [J].
Berger, S ;
Ballo, H ;
Stutte, HJ .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1996, 26 (06) :1297-1301
[6]  
BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[7]   LACK OF DOSE-RESPONSE BY DIETARY N-3 FATTY-ACIDS AT A CONSTANT RATIO OF N-3 TO N-6 FATTY-ACIDS IN SUPPRESSING EICOSANOID BIOSYNTHESIS FROM ARACHIDONIC-ACID [J].
BOUDREAU, MD ;
CHANMUGAM, PS ;
HART, SB ;
LEE, SH ;
HWANG, DH .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1991, 54 (01) :111-117
[8]  
BURDIN N, 1995, J IMMUNOL, V154, P2533
[9]   DIETARY SOURCE OF OMEGA-3-FATTY-ACIDS AFFECTS ENDOTOXIN-INDUCED PERITONEAL MACROPHAGE TUMOR-NECROSIS-FACTOR AND EICOSANOID SYNTHESIS [J].
CARRICK, JB ;
SCHNELLMANN, RG ;
MOORE, JN .
SHOCK, 1994, 2 (06) :421-426
[10]   The effect on human tumor necrosis factor alpha and interleukin 1 beta production of diets enriched in n-3 fatty acids from vegetable oil or fish oil [J].
Caughey, GE ;
Mantzioris, E ;
Gibson, RA ;
Cleland, LG ;
James, MJ .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1996, 63 (01) :116-122