Spectra Characterization of the Conformational Changes of Human Cellular Prion Protein Induced by Heparin

被引：0
|
作者：
Xiao Saijin [1 ]
Hu Pingping [1 ]
Tan Kejun [1 ]
Zhen Shujun [1 ]
Huang Chengzhi [1 ]
机构：
[1] Southwest Univ, Educ Minist, Key Lab Luminescence & Real Time Anal, Chongqing 400715, Peoples R China
来源：
ACTA CHIMICA SINICA | 2010年 / 68卷 / 10期
关键词：
prion; prion protein; glycosaminoglycans; heparin; BINDING; IDENTIFICATION; GLYCOSAMINOGLYCANS; FLUORESCENCE; TRAFFICKING; AFFINITY; FRAGMENT;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The role of glycosaminoglycans (GAGs) played in prions is still in controversy. By using heparin as an example, we investigated the interaction of GAGs and recombinant human cellular prion protein (rhPrP(23-31)(C)) by measuring the spectral features of the resonance light scattering (RLS), fluorescence and circular dichroism (CD) It was found that the intensity of both RLS and fluorescence get increased when the interaction of heparin with rhPrP(23-31)(C) occurred, with the extension of the fluorescence lifetime. Meanwhile, the CD spectra indicate that heparin could induce the conformation rich in alfa-helix change to conformation rich in beta-sheet
引用
收藏
页码：1032 / 1036
页数：5
相关论文
共 24 条
  • [1] Binding of bovine prion protein to heparin: A fluorescence polarization study
    Andrievskaia, Olga
    Potetinova, Zhanna
    Balachandran, Aru
    Nielsen, Klaus
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2007, 460 (01) : 10 - 16
  • [2] Cellular heparan sulfate participates in the metabolism of prions
    Ben-Zaken, O
    Tzaban, S
    Tal, Y
    Horonchik, L
    Esko, JD
    Vlodavsky, I
    Taraboulos, A
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (41) : 40041 - 40049
  • [3] The molecular chaperone Hsp 104 -: A molecular machine for protein disaggregation
    Boesl, Benjamin
    Grimminger, Valerie
    Walter, Stefan
    [J]. JOURNAL OF STRUCTURAL BIOLOGY, 2006, 156 (01) : 139 - 148
  • [4] The highways and byways of prion protein trafficking
    Campana, V
    Sarnataro, D
    Zurzolo, C
    [J]. TRENDS IN CELL BIOLOGY, 2005, 15 (02) : 102 - 111
  • [5] Copaxone interferes with the PrPSc-GAG interaction
    Engelstein, R.
    Ovadia, H.
    Gabizon, R.
    [J]. EUROPEAN JOURNAL OF NEUROLOGY, 2007, 14 (08) : 877 - 884
  • [6] Oligomerization of the proteolytic products is an intrinsic property of prion proteins
    Georgieva, D
    Koker, M
    Redecke, L
    Perbandt, M
    Clos, J
    Bredehorst, R
    Genov, N
    Betzel, C
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 323 (04) : 1278 - 1286
  • [7] Cell division modulates prion accumulation in cultured cells
    Ghaemmaghami, Sina
    Phuan, Puay-Wah
    Perkins, Beth
    Ullman, Julie
    May, Barnaby C. H.
    Cohen, Fred E.
    Prusiner, Stanley B.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (46) : 17971 - 17976
  • [8] Protease-resistant prion protein produced in vitro lacks detectable infectivity
    Hill, AF
    Antoniou, M
    Collinge, J
    [J]. JOURNAL OF GENERAL VIROLOGY, 1999, 80 : 11 - 14
  • [9] Fragment length influences affinity for Cu2+ and Ni2+ binding to His96 or His111 of the prion protein and spectroscopic evidence for a multiple histidine binding only at low pH
    Klewpatinond, Mark
    Viles, John H.
    [J]. BIOCHEMICAL JOURNAL, 2007, 404 : 393 - 402
  • [10] Physiology of the prion protein
    Linden, Rafael
    Martins, Vilma R.
    Prado, Marco A. M.
    Cammarota, Martin
    Izquierdo, Ivan
    Brentani, Ricardo R.
    [J]. PHYSIOLOGICAL REVIEWS, 2008, 88 (02) : 673 - 728
123