Improvement of thermostability of cold-active serine alkaline protease from the psychrotrophic bacterium Shewanella sp strain Ac10 by rational mutagenesis

被引：6

作者：

Kulakova, L

Galkin, A

Nakayama, T

Nishino, T

Esaki, N ^{[1
]}

机构：

[1] Kyoto Univ, Inst Chem Res, Uji, Kyoto 6110011, Japan

[2] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Sendai, Miyagi 9808579, Japan

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2003年 / 22卷 / 1-2期

关键词：

protease; cold-active enzymes; cold-adapted enzymes; psychrophile; thermostability; homology modeling;

D O I：

10.1016/S1381-1177(03)00012-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A serine alkaline protease (SapSh) from a psychrophilic bacterium Shewanella sp. strain Ac10 is a cold-active subtilase with low thermostability [Appl. Environ. Microbiol. 65 (1999) 611-617]. By means of homology modeling with other subtilase structures, we have constructed a mutant SapSh containing an extra salt bridge on its surface that exhibits higher thermostability and even higher V,(max)/K-m, (app) value than those of the wild-type SapSh. (C) 2003 Elsevier Science B.V. All rights reserved.

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页码：113 / 117

页数：5

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