Effect of calcium ions on the Hsp104 synthesis and heat tolerance of Saccharomyces cerevisiae

被引：3

作者：

Fedoseeva, I. V. ^{[1
]}

Varakina, N. N. ^{[1
]}

Rusaleva, T. M. ^{[1
]}

Borovskii, G. B. ^{[1
]}

Rikhvanov, E. G. ^{[1
]}

Voinikov, V. K. ^{[1
]}

机构：

[1] Russian Acad Sci, Siberian Inst Plant Physiol & Biochem, Irkutsk 664003, Russia

来源：

MICROBIOLOGY | 2010年 / 79卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

Saccharomyces cerevisiae; heat tolerance; calcium; Hsp104; mitochondria; DEPENDENT PROTEIN-KINASE; SHOCK RESPONSE; STRESS; YEAST; MITOCHONDRIA; CA2+; THERMOTOLERANCE; GENE; ARABIDOPSIS; CALCINEURIN;

D O I：

10.1134/S0026261710020049

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Effect of calcium ions on heat tolerance of Saccharomyces cerevisiae and on the induction of Hsp104 synthesis by this microorganism was studied. Short-term (30 min) treatment with CaCl2 at 30A degrees C enhanced the heat tolerance to the lethal heat shock (50A degrees C); the synthesis of Hsp104 was induced as well. The effect of Ca2+ on the heat tolerance and Hsp104 synthesis was shown to be ion-specific and was inhibited by LaCl3, which is known to block calcium ion channels on the cytoplasmic membrane. The effect of Ca2+ depended on the potential of the inner mitochondrial membrane. When the cells were treated with sodium azide, which reduced the electrochemical potential, the effect of calcium both on heat tolerance and Hsp104 synthesis was suppressed. Depending on the concentration of exogenous Ca2+ and the ambient conditions, calcium ions may either induce or inhibit the expression of the stress genes and cell viability.

引用

页码：153 / 159

页数：7

共 32 条

[1] ABNORMAL PROTEINS SERVE AS EUKARYOTIC STRESS SIGNALS AND TRIGGER THE ACTIVATION OF HEAT-SHOCK GENES [J].

ANANTHAN, J ;

GOLDBERG, AL ;

VOELLMY, R .

SCIENCE, 1986, 232 (4749) :522-524

[2]

[Anonymous], [No title captured]

[3] The hyperfluidization of mammalian cell membranes acts as a signal to initiate the heat shock protein response [J].

Balogh, G ;

Horváth, I ;

Nagy, E ;

Hoyk, Z ;

Benko, S ;

Bensaude, O ;

Vígh, L .

FEBS JOURNAL, 2005, 272 (23) :6077-6086

[4] Adaptation of the yeast Yarrowia lipolytica to heat shock [J].

Biryukova, E. N. ;

Medentsev, A. G. ;

Arinbasarova, A. Yu. ;

Akimenko, V. K. .

MICROBIOLOGY, 2007, 76 (02) :158-163

[5] Amiodarone induces a caffeine-inhibited, MID1-depedent rise in free cytoplasmic calcium in Saccharomyces cerevisiae [J].

Courchesne, WE ;

Ozturk, S .

MOLECULAR MICROBIOLOGY, 2003, 47 (01) :223-234

[6] Calcineurin signaling in Saccharomyces cereviside:: how yeast go crazy in response to stress [J].

Cyert, MS .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 311 (04) :1143-1150

[7] Hsp104 and ClpB: protein disaggregating machines [J].

Doyle, Shannon M. ;

Wickner, Sue .

TRENDS IN BIOCHEMICAL SCIENCES, 2009, 34 (01) :40-48

[8] Heat-shock-induced changes in intracellular Ca2+ level in tobacco seedlings in relation to thermotolerance [J].

Gong, M ;

van der Luit, AH ;

Knight, MR ;

Trewavas, AJ .

PLANT PHYSIOLOGY, 1998, 116 (01) :429-437

[9] ADAPTATION TO HIGH-SALT STRESS IN SACCHAROMYCES-CEREVISIAE IS REGULATED BY CA2+/CALMODULIN-DEPENDENT PHOSPHOPROTEIN PHOSPHATASE (CALCINEURIN) AND CAMP-DEPENDENT PROTEIN-KINASE [J].

HIRATA, D ;

HARADA, S ;

NAMBA, H ;

MIYAKAWA, T .

MOLECULAR & GENERAL GENETICS, 1995, 249 (03) :257-264

[10] Phosphorylation of serine 230 promotes inducible transcriptional activity of heat shock factor 1 [J].

Holmberg, CI ;

Hietakangas, V ;

Mikhailov, A ;

Rantanen, JO ;

Kallio, M ;

Meinander, A ;

Hellman, J ;

Morrice, N ;

MacKintosh, C ;

Morimoto, RI ;

Eriksson, JE ;

Sistonen, L .

EMBO JOURNAL, 2001, 20 (14) :3800-3810

← 1 2 3 4 →