Optical mapping of BAC clones from the human Y chromosome DAZ locus

被引：12
作者：
Giacalone, J
Delobette, S
Gibaja, V
Ni, L
Skiadas, Y
Qi, R
Edington, J
Lai, ZW
Gebauer, D
Zhao, HJ
Anantharaman, T
Mishra, B
Brown, LG
Saxena, R
Page, DC
Schwartz, DC [1 ]
机构：
[1] Univ Wisconsin, UW Biotechnol Ctr, Dept Genet, Madison, WI 53706 USA
[2] Univ Wisconsin, UW Biotechnol Ctr, Dept Chem, Madison, WI 53706 USA
[3] NYU, Dept Chem, WM Keck Lab Biomol Imaging, New York, NY 10003 USA
[4] NYU, Courant Inst Math Sci, Dept Comp Sci, New York, NY 10012 USA
[5] MIT, Howard Hughes Med Inst, Whitehead Inst, Cambridge, MA 02142 USA
[6] MIT, Dept Biol, Cambridge, MA 02142 USA
来源：
GENOME RESEARCH | 2000年 / 10卷 / 09期
关键词：
D O I：
10.1101/gr.112100
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The accurate mapping of clones derived from genomic regions containing complex arrangements of repeated elements presents special problems for DNA sequencers. Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC clones derived from the DAZ locus of the human Y chromosome long arm, a locus in which the entire DAZ gene as well as subsections within the gene copies have been duplicated. High-resolution optical mapping employing seven enzymes places these clones into two contigs representing four distinct copies of the DAZ gene and highlights a number of differences between individual copies of DAZ.
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收藏
页码：1421 / 1429
页数：9
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