Turn-on fluorescence probe for BSA detection and selective cell imaging

被引:33
作者
Chen, Yabei [1 ]
Li, Ke [1 ]
Zhang, Shensong [1 ]
Xu, Pan [2 ,3 ]
Song, Bo [1 ]
机构
[1] Soochow Univ, Coll Chem Chem Engn & Mat Sci, Suzhou 215123, Peoples R China
[2] Univ Siegen, Dept Chem & Biol, Sch Sci & Technol, Phys Chem 1, Adolf Reichwein Str 2, D-57076 Siegen, Germany
[3] Univ Siegen, Dept Chem & Biol, Sch Sci & Technol, Res Ctr Micro & Nanochem & Bio Technol C, Adolf Reichwein Str 2, D-57076 Siegen, Germany
基金
中国国家自然科学基金;
关键词
Turn-on fluorescence probe; Cationic amphiphile; Electrostatic interaction-driven assembly; BSA detection; Selective cell imaging; HUMAN SERUM-ALBUMIN; DIKETOPYRROLOPYRROLE; BINDING; COMPLEXES; EMISSION; BIOMOLECULES; FACILE; ION; PH;
D O I
10.1016/j.dyepig.2022.110267
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In this paper, a cationic amphiphile (denoted by DPP-10) with diketopyrrolopyrrole decorated with methoxylated tetraphenylethylene via a phenyl bridge was designed, synthesized, and applied as a turn-on fluorescence probe for bovine serum albumin (BSA) detection and selective cell imaging. DPP-10 showed very weak fluorescence emission when dispersed in the solution, while its emission was drastically enhanced upon the addition of BSA, which should be attributed to the electrostatic interaction-driven assembly between DPP-10 and BSA. The limit of detection was as low as 0.08 mu mol/L, which is lower than that reported for most probes. The fluorescence probe shows good selectivity for BSA among the tested proteins, high photostability, and immunity to the disturbance of ions. DPP-10 with excellent cytocompatibility was applied to HeLa cell imaging, where only the BSA-rich cells showed a strong signal-to-background contrast.
引用
收藏
页数:8
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