Separation of coumarins from Archangelica officinalis in high-performance liquid chromatography and thin-layer chromatography systems

被引：42
|
作者：
Hawryl, MA [1 ]
Soczewinski, E [1 ]
Dzido, TH [1 ]
机构：
[1] Med Acad, Dept Inorgan & Analyt Chem, PL-20081 Lublin, Poland
来源：
JOURNAL OF CHROMATOGRAPHY A | 2000年 / 886卷 / 1-2期
关键词：
Archangelica officinalis; coumarins;
D O I：
10.1016/S0021-9673(00)00321-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Complex, multicomponent mixtures are difficult to separate in a single chromatographic run. Therefore, the possibility to separate twelve coumarins from Archangelica officinalis was studied by combining a HPLC and a TLC system. HPLC optimized by the use of DryLab for Windows software was performed on RP-ls column and TLC was performed on silica plates. Fractions from the RP column were evaporated, applied on silica plate and developed in non-aqueous solvent. Possibilities of complete separation of investigated coumarins were discussed in RP and NP systems. The result of their complete separation was presented by HPLC chromatograms, DryLab simulated chromatograms and a video scan of TLC plate. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：75 / 81
页数：7
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