Visualizing chromatin and chromosomes in living cells

被引：73
作者：
Zink, D
Sadoni, N
Stelzer, E
机构：
[1] Univ Munich, Dept Biol 2, D-80336 Munich, Germany
[2] European Mol Biol Lab, D-69117 Heidelberg, Germany
来源：
METHODS | 2003年 / 29卷 / 01期
关键词：
live cell microscopy; live chromatin labeling; live chromosome labeling; chromosome territory;
D O I：
10.1016/S1046-2023(02)00289-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The dynamic organization of eukaryotic genomes in cell nuclei recently came into the focus of research interest. The kinetics of genome dynamics can be addressed only by approaches involving live cell microscopy. Different methods are available to visualize chromatin, specific chromatin fractions, or individual chromosome territories within nuclei of living mammalian cells. Appropriate labeling procedures as well as cell chamber systems and important controls for live cell microscopy are described. (C) 2002 Elsevier Science (USA). All rights reserved.
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收藏
页码：42 / 50
页数：9
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