Typing of Plasmodium falciparum DNA from 2 years old Giemsa-stained dried blood spots using nested polymerase chain reaction assay

被引：0

作者：

Kumar, D. ^{[1
]}

Dhiman, S. ^{[1
]}

Rabha, B. ^{[1
]}

Goswami, D. ^{[1
]}

Yadav, K. ^{[1
]}

Deka, M. ^{[2
]}

Veer, V. ^{[1
]}

Baruah, I. ^{[1
]}

机构：

[1] Def Res Lab, Med Entomol Div, Tezpur, India

[2] Gauhati Univ, Dept Biol Sci, Gauhati, Assam, India

来源：

INDIAN JOURNAL OF MEDICAL MICROBIOLOGY | 2016年 / 34卷 / 02期

关键词：

Parasitaemia; Plasmodium; polymerase chain reaction; thick blood spots; REAL-TIME PCR; MALARIA; INFECTIONS; MICROSCOPY;

D O I：

10.4103/0255-0857.176841

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A panel of 129 Giemsa-stained thick blood spots (TBS) confirmed for Plasmodium falciparum infection having different levels of parasite density were collected from a malaria endemic area. DNA was extracted and nested polymerase chain reaction (PCR) assay was performed to amplify P. falciparum DNA. Nested PCR assay successfully amplified P. falciparum DNA at a very low parasitaemia of similar to 10 parasites/mu l of blood. Current PCR assay is very simple and can be used retrospectively to monitor the invasion and prevalence of different Plasmodium species in endemic areas.

引用

页码：210 / 212

页数：3

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