SU-8-based microneedles for in vitro neural applications

被引:35
作者
Altuna, Ane [1 ]
Gabriel, Gemma [2 ,3 ]
Menendez de la Prida, Liset [4 ]
Tijero, Maria [1 ]
Guimera, Anton [2 ,3 ]
Berganzo, Javier [1 ]
Salido, Rafa [1 ,2 ]
Villa, Rosa
Fernandez, Luis J. [1 ]
机构
[1] Ikerlan S Coop, MEMS MST Dept, Arrasate Mondragon 20500, Spain
[2] CSIC, CNM, Inst Microelect Barcelona, Barcelona 08913, Spain
[3] Ctr Invest Biomed Red Biomat & Nanomed CIBER BBN, Zaragoza, Spain
[4] CSIC, Inst Cajal, E-28002 Madrid, Spain
关键词
MICROFLUIDIC CHANNELS; DRUG-DELIVERY; MICROELECTRODES; PROBES; IMPLANT; DEVICES;
D O I
10.1088/0960-1317/20/6/064014
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
This paper presents novel design, fabrication, packaging and the first in vitro neural activity recordings of SU-8-based microneedles. The polymer SU-8 was chosen because it provides excellent features for the fabrication of flexible and thin probes. A microprobe was designed in order to allow a clean insertion and to minimize the damage caused to neural tissue during in vitro applications. In addition, a tetrode is patterned at the tip of the needle to obtain fine-scale measurements of small neuronal populations within a radius of 100 mu m. Impedance characterization of the electrodes has been carried out to demonstrate their viability for neural recording. Finally, probes are inserted into 400 mu m thick hippocampal slices, and simultaneous action potentials with peak-to-peak amplitudes of 200-250 mu V are detected.
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页数:6
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