Antioxidant activities of water-soluble polysaccharide extracted from mung bean (Vigna radiata L.) hull with ultrasonic assisted treatment

被引：192

作者：

Lai, Furao ^{[1
]}

Wen, Qibiao ^{[1
]}

Li, Lin ^{[1
]}

Wu, Hui ^{[1
]}

Li, Xiaofeng ^{[1
]}

机构：

[1] S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 81卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Mung bean; Water-soluble polysaccharide; Ultrasonic; Antioxidant; RADICAL-SCAVENGING ACTIVITIES; OXIDATIVE DAMAGE; ASSAY; IDENTIFICATION; MECHANISMS; FRACTIONS; STRESS;

D O I：

10.1016/j.carbpol.2010.02.011

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Ultrasonic assisted extraction and antioxidant activity of water-soluble polysaccharide (MSP) from mung bean (Vigna radiata L) hull were investigated. After deproteinization, MSP was isolated and purified by a DEAE-cellulose anion-exchange column and a Sephadex G-100 gel-permeation column. Two acid polysaccharide fractions, MP1 and MP2, were characterized by FT-IR, GC, and gel-permeation chromatography (GPC). Results indicated that MP1 and MP2 were acid heteropolysaccharide with 9.9% and 36.4% of uronic acid contents respectively. The main composition of MP1 was mannose, whereas MP2 consisted of rhamnose and galactose. The molecule weights of MP1 and MP2 were 83 kDa and 45 kDa, respectively. Although, MP2 showed higher hydroxyl radical-scavenging activity, MP1 exhibited higher reducing power and stronger scavenging capacity for superoxide radical and DPPH radicals as well as higher inhibition on self-oxidation of 1,2,3-phentriol than that of MP2. Thus, MP1 should be explored as a potential antioxidant. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：323 / 329

页数：7

共 47 条

[1] Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies [J].

Amarowicz, R ;

Pegg, RB ;

Rahimi-Moghaddam, P ;

Barl, B ;

Weil, JA .

FOOD CHEMISTRY, 2004, 84 (04) :551-562

[2] Wild rice mull antioxidants [J].

Asamarai, AM ;

Addis, PB ;

Epley, RJ ;

Krick, TP .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1996, 44 (01) :126-130

[3] ANTIOXIDANT DETERMINATIONS BY THE USE OF A STABLE FREE RADICAL [J].

BLOIS, MS .

NATURE, 1958, 181 (4617) :1199-1200

[4] Antioxidants, oxidative damage and oxygen deprivation stress: a review [J].

Blokhina, O ;

Virolainen, E ;

Fagerstedt, KV .

ANNALS OF BOTANY, 2003, 91 (02) :179-194

[5]

Blunenkrantz N., 1973, ANAL BIOCHEM, V54, P484

[6]

BOMER FRJ, 1989, AM J CLIN NUTR, V50, P315

[7]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[8] Biological activities of sulfated polysaccharides from tropical seaweeds [J].

Costa, L. S. ;

Fidelis, G. P. ;

Cordeiro, S. L. ;

Oliveira, R. M. ;

Sabry, D. A. ;

Camara, R. B. G. ;

Nobre, L. T. D. B. ;

Costa, M. S. S. P. ;

Almeida-Lima, J. ;

Farias, E. H. C. ;

Leite, E. L. ;

Rocha, H. A. O. .

BIOMEDICINE & PHARMACOTHERAPY, 2010, 64 (01) :21-28

[9] Oxidative damage to DNA in diabetes mellitus [J].

Dandona, P ;

Thusu, K ;

Cook, S ;

Snyder, B ;

Makowski, J ;

Armstrong, D ;

Nicotera, T .

LANCET, 1996, 347 (8999) :444-445

[10]

DISCHE Z, 1947, J BIOL CHEM, V167, P189

← 1 2 3 4 5 →