Mechanisms and Strategies for Determining m6A RNA Modification Sites by Natural and Engineered m6A Effector Proteins

被引：13

作者：

Imanishi, Miki ^{[1
]}

机构：

[1] Kyoto Univ, Inst Chem Res, Uji, Kyoto 6110011, Japan

来源：

CHEMISTRY-AN ASIAN JOURNAL | 2022年 / 17卷 / 16期

基金：

日本学术振兴会;

关键词：

RNA methylation; RNA recognition; N-6-metyladenosine; Protein engineering; Gene expression; MESSENGER-RNA; G-QUADRUPLEX; N-6-METHYLADENOSINE RNA; CRYSTAL-STRUCTURE; NONCODING RNA; BINDING; METHYLATION; RECOGNITION; REVEALS; CELLS;

D O I：

10.1002/asia.202200367

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

N-6-Methyladenosine (m(6)A) is the most common internal RNA modification in the consensus sequence of 5 '-RRACH-3 '. The methyl mark is added by writer proteins (METTL3/METTL14 metyltransferase complex) and removed by eraser proteins (m(6)A demethylases; FTO and ALKBH5). Recognition of this methyl mark by m(6)A reader proteins leads to changes in RNA metabolism. How the writer and eraser proteins determine their targets is not well-understood, despite the importance of this information in understanding the regulatory mechanisms and physiological roles of m(6)A. However, approaches for targeted manipulation of the methylation state at specific sites are being developed. In this review, I summarize the recent findings on the mechanisms of target identification of m(6)A regulatory proteins, as well as recent approaches for targeted m(6)A modifications.

引用

页数：10

共 120 条

[1] Engineering reprogrammable RNA-binding proteins for study and manipulation of the transcriptome [J].

Abil, Zhanar ;

Zhao, Huimin .

MOLECULAR BIOSYSTEMS, 2015, 11 (10) :2658-2665

[2] Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA [J].

Abil, Zhanar ;

Denard, Carl A. ;

Zhao, Huimin .

JOURNAL OF BIOLOGICAL ENGINEERING, 2014, 8

[3] A cytosine deaminase for programmable single-base RNA editing [J].

Abudayyeh, Omar O. ;

Gootenberg, Jonathan S. ;

Franklin, Brian ;

Koob, Jeremy ;

Kellner, Max J. ;

Ladha, Alim ;

Joung, Julia ;

Kirchgatterer, Paul ;

Cox, David B. T. ;

Zhang, Feng .

SCIENCE, 2019, 365 (6451) :382-+

[4] RNA targeting with CRISPR-Cas13 [J].

Abudayyeh, Omar O. ;

Gootenberg, Jonathan S. ;

Essletzbichler, Patrick ;

Han, Shuo ;

Joung, Julia ;

Belanto, Joseph J. ;

Verdine, Vanessa ;

Cox, David B. T. ;

Kellner, Max J. ;

Regev, Aviv ;

Lander, Eric S. ;

Voytas, Daniel F. ;

Ting, Alice Y. ;

Zhang, Feng .

NATURE, 2017, 550 (7675) :280-+

[5] C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector [J].

Abudayyeh, Omar O. ;

Gootenberg, Jonathan S. ;

Konermann, Silvana ;

Joung, Julia ;

Slaymaker, Ian M. ;

Cox, David B. T. ;

Shmakov, Sergey ;

Makarova, Kira S. ;

Semenova, Ekaterina ;

Minakhin, Leonid ;

Severinov, Konstantin ;

Regev, Aviv ;

Lander, Eric S. ;

Koonin, Eugene V. ;

Zhang, Feng .

SCIENCE, 2016, 353 (6299)

[6] Programmable RNA-binding protein composed of repeats of a single modular unit [J].

Adamala, Katarzyna P. ;

Martin-Alarcon, Daniel A. ;

Boyden, Edward S. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (19) :E2579-E2588

[7] Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control [J].

Barbieri, Isaia ;

Tzelepis, Konstantinos ;

Pandolfini, Luca ;

Shi, Junwei ;

Millan-Zambrano, Gonzalo ;

Robson, Samuel C. ;

Aspris, Demetrios ;

Migliori, Valentina ;

Bannister, Andrew J. ;

Han, Namshik ;

De Braekeleer, Etienne ;

Ponstingl, Hannes ;

Hendrick, Alan ;

Vakoc, Christopher R. ;

Vassiliou, George S. ;

Kouzarides, Tony .

NATURE, 2017, 552 (7683) :126-+

[8] m6A RNA Modification Controls Cell Fate Transition in Mammalian Embryonic Stem Cells [J].

Batista, Pedro J. ;

Molinie, Benoit ;

Wang, Jinkai ;

Qu, Kun ;

Zhang, Jiajing ;

Li, Lingjie ;

Bouley, Donna M. ;

Lujan, Ernesto ;

Haddad, Bahareh ;

Daneshvar, Kaveh ;

Carter, Ava C. ;

Flynn, Ryan A. ;

Zhou, Chan ;

Lim, Kok-Seong ;

Dedon, Peter ;

Wernig, Marius ;

Mullen, Alan C. ;

Xing, Yi ;

Giallourakis, Cosmas C. ;

Chang, Howard Y. .

CELL STEM CELL, 2014, 15 (06) :707-719

[9] The SMAD2/3 interactome reveals that TGFβ controls m6A mRNA methylation in pluripotency [J].

Bertero, Alessandro ;

Brown, Stephanie ;

Madrigal, Pedro ;

Osnato, Anna ;

Ortmann, Daniel ;

Yiangou, Loukia ;

Kadiwala, Juned ;

Hubner, Nina C. ;

de los Mozos, Igor Ruiz ;

Sadee, Christoph ;

Lenaerts, An-Sofie ;

Nakanoh, Shota ;

Grandy, Rodrigo ;

Farnell, Edward ;

Ule, Jernej ;

Stunnenberg, Hendrik G. ;

Mendjan, Sasha ;

Vallier, Ludovic .

NATURE, 2018, 555 (7695) :256-+

[10] MODOMICS: a database of RNA modification pathways. 2021 update [J].

Boccaletto, Pietro ;

Stefaniak, Filip ;

Ray, Angana ;

Cappannini, Andrea ;

Mukherjee, Sunandan ;

Purta, Elzbieta ;

Kurkowska, Malgorzata ;

Shirvanizadeh, Niloofar ;

Destefanis, Eliana ;

Groza, Paula ;

Avsar, Gulben ;

Romitelli, Antonia ;

Pir, Pinar ;

Dassi, Erik ;

Conticello, Silvestro G. ;

Aguilo, Francesca ;

Bujnicki, Janusz M. .

NUCLEIC ACIDS RESEARCH, 2022, 50 (D1) :D231-D235

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