Monolayer-controlled substrate selectivity using noncovalent enzyme-nanopartide conjugates

被引：93
作者：
Hong, R
Emrick, T
Rotello, VM [1 ]
机构：
[1] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA
[2] Univ Massachusetts, Dept Polymer Sci & Engn, Amherst, MA 01003 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 42期
关键词：
D O I：
10.1021/ja0461163
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Electrostatic interactions were used to noncovalently conjugate chymotrypsin to gold nanoparticles featuring hybrid tetraethylene(glycol)alkanethiol monolayers terminated with carboxylate groups. This conjugation process greatly alters the substrate selectivity of the adsorbed chymotrypsin, inhibiting the hydrolysis of anionic subtrates without affecting the hydrolysis rate of cationic analogues. Copyright © 2004 American Chemical Society.
引用
收藏
页码：13572 / 13573
页数：2
相关论文
共 26 条
[1]   Proteases in organic synthesis [J].
Bordusa, F .
CHEMICAL REVIEWS, 2002, 102 (12) :4817-4867
[2]   Immobilizing enzymes: How to create more suitable biocatalysts [J].
Bornscheuer, UT .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (29) :3336-3337
[3]   Immobilised enzymes: carrier-bound or carrier-free? [J].
Cao, LQ ;
van Langen, L ;
Sheldon, RA .
CURRENT OPINION IN BIOTECHNOLOGY, 2003, 14 (04) :387-394
[4]   Mechanistic origins of the substrate selectivity of serine proteases [J].
Case, A ;
Stein, RL .
BIOCHEMISTRY, 2003, 42 (11) :3335-3348
[5]   Redox properties of cytochrome c adsorbed on self-assembled monolayers:: A probe for protein conformation and orientation [J].
Chen, XX ;
Ferrigno, R ;
Yang, J ;
Whitesides, GA .
LANGMUIR, 2002, 18 (18) :7009-7015
[6]   Chemical modification of biocatalysts [J].
Davis, BG .
CURRENT OPINION IN BIOTECHNOLOGY, 2003, 14 (04) :379-386
[7]   Inhibition of chymotrypsin through surface binding using nanoparticle-based receptors [J].
Fischer, NO ;
McIntosh, CM ;
Simard, JM ;
Rotello, VM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (08) :5018-5023
[8]   Pepsin-gold colloid conjugates: Preparation, characterization, and enzymatic activity [J].
Gole, A ;
Dash, C ;
Ramakrishnan, V ;
Sainkar, SR ;
Mandale, AB ;
Rao, M ;
Sastry, M .
LANGMUIR, 2001, 17 (05) :1674-1679
[9]   Chemical engineering of enzymes:: Altered catalytic activity, predictable selectivity and exceptional stability of the semisynthetic peroxidase seleno-subtilisin [J].
Häring, D ;
Schreier, P .
NATURWISSENSCHAFTEN, 1999, 86 (07) :307-312
[10]  
Häring D, 1998, ANGEW CHEM INT EDIT, V37, P2471, DOI 10.1002/(SICI)1521-3773(19981002)37:18<2471::AID-ANIE2471>3.0.CO
123