A new approach for efficient regeneration of a recalcitrant genotype of sunflower (Helianthus annuus) by organogenesis induction on split embryonic

被引：12

作者：

Hewezi, T ^{[1
]}

Jardinaud, F ^{[1
]}

Alibert, G ^{[1
]}

Kallerhoff, J ^{[1
]}

机构：

[1] INP ENSAT, Lab Biotechnol & Ameliorat Plantes, F-31326 Castanet Tolosan, France

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2003年 / 73卷 / 01期

关键词：

adventitious shoot regeneration; AFLP; genetic stability; Helianthus annuus L;

D O I：

10.1023/A:1022689229547

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new approach for shoot regeneration from split embryonic axes of Helianthus annuus var. RHA266, a poor responding genotype to in vitro culture, has been developed. The regeneration procedure is based on successive excision of the apical and axillary shoots originating from pre-existing meristems. This elimination process stimulates adventitious shoot bud formation in 80% of treated explants. Although newly induced buds were observed on hormone-free medium, higher rate of induction and bud development were obtained on medium containing 0.1 mg l(-1) BA. All regenerated plants were phenotypically similar to seed-derived RHA266 plants with no observed somaclonal variation, as assessed by AFLP analysis. The efficient shoot regeneration from newly formed meristematic cells should offer opportunities to genetically engineer economically important sunflower genotypes that have proven till now, to be recalcitrant towards other regeneration systems.

引用

页码：81 / 86

页数：6

共 30 条

[21] SHOOT TIP CULTURE OF HELIANTHUS-ANNUUS FLOWERING AND DEVELOPMENT OF ADVENTITIOUS AND MULTIPLE SHOOTS [J].

PATERSON, KE .

AMERICAN JOURNAL OF BOTANY, 1984, 71 (07) :925-931

[22] ORGANOGENESIS FROM HELIANTHUS-ANNUUS INBREDS AND HYBRIDS FROM THE COTYLEDONS OF ZYGOTIC EMBRYOS [J].

POWER, CJ .

AMERICAN JOURNAL OF BOTANY, 1987, 74 (04) :497-503

[23] GENETIC-TRANSFORMATION BY AGROBACTERIUM-TUMEFACIENS IN THE INTERSPECIFIC HYBRID HELIANTHUS-ANNUUS X HELIANTHUS-TUBEROSUS [J].

PUGLIESI, C ;

BIASINI, MG ;

FAMBRINI, M ;

BARONCELLI, S .

PLANT SCIENCE, 1993, 93 (1-2) :105-115

[24] ORGANOGENESIS AND EMBRYOGENESIS IN HELIANTHUS-TUBEROSUS AND IN THE INTERSPECIFIC HYBRID HELIANTHUS-ANNUUS X HELIANTHUS-TUBEROSUS [J].

PUGLIESI, C ;

MEGALE, P ;

CECCONI, F ;

BARONCELLI, S .

PLANT CELL TISSUE AND ORGAN CULTURE, 1993, 33 (02) :187-193

[25] CALLUS DEVELOPMENT AND PLANT-REGENERATION FROM DIFFERENT EXPLANTS OF 6 SPECIES OF SUNFLOWER (HELIANTHUS L) [J].

PUNIA, MS ;

BOHOROVA, NE .

PLANT SCIENCE, 1992, 87 (01) :79-83

[26]

ROSELAND CR, 1991, EUPHYTICA, V54, P183

[27] In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets [J].

Tang, W .

PLANT CELL REPORTS, 2001, 20 (02) :163-168

[28] The genetics of resistance to five races of downy mildew (Plasmopara halstedii) in sunflower (Helianthus annuus L.) [J].

Vear, F ;

Gentzbittel, L ;

Philippon, J ;

Mouzeyar, S ;

Mestries, E ;

RoeckelDrevet, P ;

deLabrouhe, DT ;

Nicolas, P .

THEORETICAL AND APPLIED GENETICS, 1997, 95 (04) :584-589

[29] AFLP analysis of variation in pecan somatic embryos [J].

Vendrame, WA ;

Kochert, G ;

Wetzstein, HY .

PLANT CELL REPORTS, 1999, 18 (10) :853-857

[30] In vitro propagation, restriction fragment length polymorphism, and random amplified polymorphic DNA analyses of Angelica plants [J].

Watanabe, A ;

Araki, S ;

Kobari, S ;

Sudo, H ;

Tsuchida, T ;

Uno, T ;

Kosaka, N ;

Shimomura, K ;

Yamazaki, M ;

Saito, K .

PLANT CELL REPORTS, 1998, 18 (3-4) :187-192

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