Primary structure and functional activity of the Bacillus subtilis gene ribC

被引：0
作者：
Gusarov, II [1 ]
Kreneva, RA
Rybak, KV
Podchernyaev, DA
Iomantas, YV
Kolibaba, LG
Polanuer, BM
Kozlov, YI
Perumov, DA
机构：
[1] Moscow Genet & Select Ind Microorganisms Inst, Moscow 113545, Russia
[2] Russian Acad Sci, Inst Nucl Phys, Gatchina 188350, Russia
来源：
MOLECULAR BIOLOGY | 1997年 / 31卷 / 05期
关键词：
Bacillus subtilis; riboflavin operon; regulatory gene; primary structure;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ribC gene controlling the riboflavin operon in Bacillus subtilis was cloned by the resistance to a riboflavin analog 7,8-dimethyl-10-(O-methylcetoxime)-isoalloxazine. The homology was revealed with the riboflavin kinase/FAD synthase gene from Corynebacrerium ammoniagenes and Escherichia coli. Riboflavin kinase/FAD synthase activity was detected in cell-free extracts, thus demonstrating that the ribC gene codes for a bifunctional enzyme producing FAD from riboflavin via FMN.
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页码：698 / 703
页数：6
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