Sexual dimorphism and regulation of resistin, adiponectin, and leptin expression in the mouse

被引:114
作者
Gui, YT
Silha, JV
Murphy, LJ
机构
[1] Univ Manitoba, Dept Physiol & Med, Winnipeg, MB R3E 0W3, Canada
[2] Peking Univ, Shenzhen Hosp, Ctr Reprod Med, Shenzhen, Peoples R China
来源
OBESITY RESEARCH | 2004年 / 12卷 / 09期
关键词
sexual dimorphism; resistin; adiponectin; leptin; estrous cycle;
D O I
10.1038/oby.2004.185
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: To examine gender differences and hormonal regulation of resistin, adiponectin, and leptin. Research Methods and Procedures: Plasma levels were measured, and mRNA expression in perigonadal fat was quantified by RNase protection assays. Results: Plasma resistin declined with age despite an increase in adiposity in both genders. In male mice, plasma leptin increased, whereas adiponectin levels were constant. In females, both adiponectin and leptin levels increased with age. Resistin mRNA levels were significantly higher in female than male mice at all ages, whereas leptin and adiponectin mRNA levels were similar in fat from 6-week-old male and female mice, and sexual dimorphism was apparent only in the older mice, with higher levels apparent in females. Castration did not abolish gender differences in plasma levels or resistin, adiponectin, or leptin mRNAs. Castration of male mice did not significantly change adipokine mRNA levels or plasma levels of resistin or leptin; however, adiponectin was significantly increased. Dihydrotestosterone treatment had no effect on adipokine mRNA expression or resistin and adiponectin levels but increased leptin levels. In contrast, ovariectomy significantly increased resistin mRNA abundance and decreased leptin and adiponectin mRNAs. Plasma leptin levels were also increased by ovariectomy, whereas resistin and adiponectin levels were unchanged. Estrogen replacement significantly reduced resistin mRNA and increased leptin and adiponectin mRNA levels but had no effect on plasma adipokine levels. Discussion: The gender differences in adipokine mRNA expression and plasma levels were not ablated by castration and seem to be dependent on other factors in addition to gonadal steroids.
引用
收藏
页码:1481 / 1491
页数:11
相关论文
共 33 条
[1]   GLUCOSE-TOLERANCE AND PLASMA-INSULIN OF RAT IN RELATION TO ESTROUS-CYCLE AND SEX-HORMONES [J].
BAILEY, CJ ;
MATTY, AJ .
HORMONE AND METABOLIC RESEARCH, 1972, 4 (04) :266-&
[2]   Analysis of paradoxical observations on the association between leptin and insulin resistance [J].
Ceddia, RB ;
Koistinen, HA ;
Zierath, JR ;
Sweeney, G .
FASEB JOURNAL, 2002, 16 (10) :1163-1176
[3]   Plasma leptin, fatty acids, and tumor necrosis factor-receptor and insulin resistance in children [J].
Chu, NF ;
Chang, JB ;
Shieh, SM .
OBESITY RESEARCH, 2003, 11 (04) :532-540
[4]   Sexual differentiation, pregnancy, calorie restriction, and aging affect the adipocyte-specific secretory protein adiponectin [J].
Combs, TP ;
Berg, AH ;
Rajala, MW ;
Klebanov, S ;
Iyengar, P ;
Jimenez-Chillaron, JC ;
Patti, ME ;
Klein, SL ;
Weinstein, RS ;
Scherer, PE .
DIABETES, 2003, 52 (02) :268-276
[5]   Hexosamines and nutrient excess induce leptin production and leptin receptor activation in pancreatic islets and clonal β-cells [J].
Emilsson, V ;
O'Dowd, J ;
Nolan, AL ;
Cawthorne, MA .
ENDOCRINOLOGY, 2001, 142 (10) :4414-4419
[6]   What's in a name? In search of leptin's physiologic role [J].
Flier, JS .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1998, 83 (05) :1407-1413
[7]   Control of energy homeostasis and insulin action by adipocyte hormones: leptin, acylation stimulating protein, and adiponectin [J].
Havel, PJ .
CURRENT OPINION IN LIPIDOLOGY, 2002, 13 (01) :51-59
[8]   AdipoQ is a novel adipose-specific gene dysregulated in obesity [J].
Hu, E ;
Liang, P ;
Spiegelman, BM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (18) :10697-10703
[9]   Adiponectin expression from human adipose tissue -: Relation to obesity, insulin resistance, and tumor necrosis factor-α expression [J].
Kern, PA ;
Di Gregorio, GB ;
Lu, T ;
Rassouli, N ;
Ranganathan, G .
DIABETES, 2003, 52 (07) :1779-1785
[10]   Regulation of leptin by steroid hormones in rat adipose tissue [J].
Kristensen, K ;
Pedersen, SB ;
Richelsen, B .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 259 (03) :624-630