Purification of cytochrome b-561 from bean hypocotyls plasma membrane.: Evidence for the presence of two heme centers

被引:26
作者
Trost, P
Bèrczi, A
Sparla, F
Sponza, G
Marzadori, B
Asard, H
Pupillo, P
机构
[1] Univ Bologna, Dept Biol, I-40126 Bologna, Italy
[2] Hungarian Acad Sci, Biol Res Ctr, Inst Biophys, H-6701 Szeged, Hungary
[3] Univ Antwerp, RUCA, Dept Biol, B-2020 Antwerp, Belgium
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2000年 / 1468卷 / 1-2期
关键词
cytochrome b-561; ascorbate; plasma membrane redox; redox titration; Phaseolus vulgaris;
D O I
10.1016/S0005-2736(00)00283-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The high potential, ascorbate-reducible b-type cytochrome of plant plasma membranes, named cytochrome b-561, has been purified to homogeneity from etiolated bean hypocotyls. The pure protein migrated in denaturing electrophoresis as a broad band of approximately 55 kDa, and was found to be glycosylated. Optical redox titrations of partially purified cytochrome b-561 indicated that it contains two hemes with similar spectral features, but distinct midpoint redox potentials (E-m7+135 mV and +206 mV, respectively). The presence of two heme centers in cytochrome b-561 is consistent with its role in electron transfer across plant plasma membranes. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:1 / 5
页数:5
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