Two-photon intravital imaging of thrombus development

被引：53

作者：

Kamocka, Malgorzata M. ^{[1
,2
]}

Mu, Jian ^{[3
]}

Liu, Xiaomin ^{[3
]}

Chen, Nan ^{[4
]}

Zollman, Amy ^{[1
,2
]}

Sturonas-Brown, Barbara ^{[5
]}

Dunn, Kenneth ^{[5
]}

Xu, Zhiliang ^{[6
]}

Chen, Danny Z. ^{[3
]}

Alber, Mark S. ^{[6
]}

Rosen, Elliot D. ^{[1
,2
]}

机构：

[1] Indiana Univ Sch Med, Dept Med & Mol Genet, Indianapolis, IN 46202 USA

[2] Indiana Ctr Vasc Biol & Med, Indianapolis, IN 46202 USA

[3] Univ Notre Dame, Dept Comp Sci & Engn, Notre Dame, IN 46556 USA

[4] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA

[5] Indiana Univ Sch Med, Indiana Ctr Biol Microscopy, Indianapolis, IN 46202 USA

[6] Univ Notre Dame, Dept Math, Interdisciplinary Ctr Study Biocomplex, Notre Dame, IN 46556 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2010年 / 15卷 / 01期

基金：

美国国家科学基金会;

关键词：

thrombogenesis; coagulation; hemostasis; laser vascular injury; two-photon imaging; intravital microscopy; IN-VIVO; MOUSE MODELS; MURINE MODELS; LIVING MOUSE; P-SELECTIN; MICROSCOPY; MICE; ATHEROSCLEROSIS; HEMOSTASIS; THROMBOEMBOLISM;

D O I：

10.1117/1.3322676

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Thrombus development in mouse mesenteric vessels following laser-induced injury was monitored by high-resolution, near-real-time, two-photon, intravital microscopy. In addition to the use of fluorescently tagged fibrin(ogen) and platelets, plasma was labeled with fluorescently tagged dextran. Because blood cells exclude the dextran in the single plane, blood cells appear as black silhouettes. Thus, in addition to monitoring the accumulation of platelets and fibrin in the thrombus, the protocol detects the movement and incorporation of unlabeled cells in and around it. The developing thrombus perturbs the blood flow near the thrombus surface, which affects the incorporation of platelets and blood cells into the structure. The hemodynamic effects and incorporation of blood cells lead to the development of thrombi with heterogeneous domain structures. Additionally, image processing algorithms and simulations were used to quantify structural features of developing thrombi. This analysis suggests a novel mechanism to stop the growth of developing thrombus. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3322676]

引用

页数：7

共 38 条

[1] Links between adipose tissue and thrombosis in the mouse [J].

Bodary, Peter F. .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2007, 27 (11) :2284-2291

[2] Thrombus formation: direct real-time observation and digital analysis of thrombus assembly in a living mouse by confocal and widefield intravital microscopy [J].

Celi, A ;

Merrill-Skoloff, G ;

Gross, P ;

Falati, S ;

Sim, DS ;

Flaumenhaft, R ;

Furie, BC ;

Furie, B .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2003, 1 (01) :60-68

[3] Molecular, cellular and functional imaging of atherothrombosis [J].

Choudhury, RP ;

Fuster, V ;

Fayad, ZA .

NATURE REVIEWS DRUG DISCOVERY, 2004, 3 (11) :913-925

[4]

Cina Gregorio, 1996, Rays (Rome), V21, P315

[5] Voxx: a PC-based, near real-time volume rendering system for biological microscopy [J].

Clendenon, JL ;

Phillips, CL ;

Sandoval, RM ;

Fang, SF ;

Dunn, KW .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2002, 282 (01) :C213-C218

[6] Increased venous versus arterial thrombosis in the Factor V Leiden mouse [J].

Cooley, Brian C. ;

Chen, Chao-Ying ;

Schmeling, Gregory .

THROMBOSIS RESEARCH, 2007, 119 (06) :747-751

[7] Murine thrombosis models [J].

Day, SM ;

Reeve, JL ;

Myers, DD ;

Fay, WP .

THROMBOSIS AND HAEMOSTASIS, 2004, 92 (03) :486-494

[8] Inflammation in atherosclerosis - Visualizing matrix metalloproteinase action in macrophages in vivo [J].

Deguchi, Jun-o ;

Aikawa, Masanori ;

Tung, Ching-Hsuan ;

Aikawa, Elena ;

Kim, Dong-Eog ;

Ntziachristos, Vasilis ;

Weissleder, Ralph ;

Libby, Peter .

CIRCULATION, 2006, 114 (01) :55-62

[9] A mouse model of severe von Willebrand disease:: Defects in hemostasis and thrombosis [J].

Denis, C ;

Methia, N ;

Frenette, PS ;

Rayburn, H ;

Ullman-Culleré, M ;

Hynes, RO ;

Wagner, DD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (16) :9524-9529

[10] Real-time in vivo imaging of platelets, tissue factor and fibrin during arterial thrombus formation in the mouse [J].

Falati, S ;

Gross, P ;

Merrill-Skoloff, G ;

Furie, BC ;

Furie, B .

NATURE MEDICINE, 2002, 8 (10) :1175-1180

← 1 2 3 4 →