Nanomechanics of G-quadruplexes within the promoter of the KIT oncogene

被引：13

作者：

Buglione, Enrico ^{[1
]}

Salerno, Domenico ^{[1
]}

Marrano, Claudia Adriana ^{[1
]}

Cassina, Valeria ^{[1
]}

Vesco, Guglielmo ^{[1
,5
]}

Nardo, Luca ^{[1
,5
]}

Dacasto, Mauro ^{[2
]}

Rigo, Riccardo ^{[3
]}

Sissi, Claudia ^{[3
,4
]}

Mantegazza, Francesco ^{[1
]}

机构：

[1] Univ Milano Bicocca, BioNanoMed Ctr NANOMIB, Sch Med & Surg, I-20854 Vedano Al Lambro, MB, Italy

[2] Univ Padua, Dept Comparat Biomed & Food Sci, I-35020 Legnaro, PD, Italy

[3] Univ Padua, Dept Pharmaceut & Pharmacol Sci, I-35131 Padua, PD, Italy

[4] Univ Padua, Interdept Res Ctr Innovat Biotechnol CRIBI, I-35121 Padua, PD, Italy

[5] Univ Insubria, Dept Sci & High Technol, I-22100 Como, CO, Italy

来源：

NUCLEIC ACIDS RESEARCH | 2021年 / 49卷 / 08期

关键词：

MAGNETIC TWEEZERS MEASUREMENTS; SINGLE-MOLECULE; SUPERCOILED DNA; I-MOTIF; STABILITY; FORCE; QUANTIFICATION; KINETICS; REVEALS; RNA;

D O I：

10.1093/nar/gkab079

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

G-quadruplexes (G4s) are tetrahelical DNA structures stabilized by four guanines paired via Hoogsteen hydrogen bonds into quartets. While their presence within eukaryotic DNA is known to play a key role in regulatory processes, their functional mechanisms are still under investigation. In the present work, we analysed the nanomechanical properties of three G4s present within the promoter of the KIT proto-oncogene from a single-molecule point of view through the use of magnetic tweezers (MTs). The study of DNA extension fluctuations under negative supercoiling allowed us to identify a characteristic fingerprint of G4 folding. We further analysed the energetic contribution of G4 to the double-strand denaturation process in the presence of negative supercoiling, and we observed a reduction in the energy required for strands separation.

引用

页码：4564 / 4573

页数：10

共 65 条

[1] Stretched and overwound DNA forms a Pauling-like structure with exposed bases [J].