Partial purification of α-amylase from culture supernatant of Bacillus subtilis in aqueous two-phase systems

被引：26
|
作者：
Zhi, WB [1 ]
Song, JM [1 ]
Bi, JX [1 ]
Fan, OY [1 ]
机构：
[1] Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
来源：
BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2004年 / 27卷 / 01期
关键词：
alpha-amylase; aqueous two-phase systems; purification;
D O I：
10.1007/s00449-004-0369-x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A study was made of the partition and purification of alpha-amylase from a culture supernatant of Bacillus subtilis in the polyethylene glycol (PEG)-citrate aqueous two-phase system (ATPS). Factors that influenced the partition of the protein in this system, including the molecular weight of the PEG, the tie line length of ATPS, the pH value and the sodium chloride concentration, were investigated. Purification of alpha-amylase was attained with a purification factor (PF) of 1.8 and 90% yield at pH 6.0 in a PEG1000-citrate ATPS with short tie line length. By utilizing the salt-out effect of neutral salt, the purification of alpha-amylase was further improved to 2.0 of PF and 80% yield in a PEG3350-citrate ATPS with 4% sodium chloride.
引用
收藏
页码：3 / 7
页数：5
相关论文
共 50 条
  • [1] Partial purification of α-amylase from culture supernatant of Bacillus subtilis in aqueous two-phase systems
    Wenbo Zhi
    Jiangnan Song
    Jingxiu Bi
    Fan Ouyang
    Bioprocess and Biosystems Engineering, 2004, 27 : 3 - 7
  • [2] Alpha-amylase production in aqueous two-phase systems by Bacillus subtilis
    Konsoula, Z
    Liakopoulou-Kyriakides, M
    FEBS JOURNAL, 2005, 272 : 497 - 497
  • [3] One-step purification of α-amylase from the cultivation supernatant of recombinant Bacillus subtilis by high-speed counter-current chromatography with aqueous polymer two-phase systems
    Zhi, WB
    Deng, QY
    Song, JG
    Gu, M
    Ouyang, F
    JOURNAL OF CHROMATOGRAPHY A, 2005, 1070 (1-2) : 215 - 219
  • [4] Purification of extracellular α-amylase from Bacillus subtilis by partitioning in a polyethylene glycol/potassium phosphate aqueous two-phase system
    Nguyen Hoang Loc
    Nguyen Thi Thuy Mien
    Do Thi Bich Thuy
    ANNALS OF MICROBIOLOGY, 2010, 60 (04) : 623 - 628
  • [5] Purification of extracellular α-amylase from Bacillus subtilis by partitioning in a polyethylene glycol/potassium phosphate aqueous two-phase system
    Nguyen Hoang Loc
    Nguyen Thi Thuy Mien
    Do Thi Bich Thuy
    Annals of Microbiology, 2010, 60 : 623 - 628
  • [6] Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process
    Rosa, Paula A. J.
    Azevedo, Ana M.
    Sommerfeld, S.
    Mutter, Martina
    Baecker, Werner
    Aires-Barros, M. Raquel
    BIOTECHNOLOGY JOURNAL, 2013, 8 (03) : 352 - 362
  • [7] α-Amylase production in aqueous two-phase systems with Bacillus amyloliquefaciens
    Celal Ülger
    Çervin Çırakoğlu
    World Journal of Microbiology and Biotechnology, 2001, 17 : 553 - 555
  • [8] α-amylase production in aqueous two-phase systems with Bacillus amyloliquefaciens
    Ülger, C
    Çirakoglu, Ç
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2001, 17 (06): : 553 - 555
  • [9] Aqueous Two-Phase Systems for the Isolation and Partial Purification of Lipases from Soil Bacteria
    Saddique, Hark
    Aasim, Muhammad
    Nawab, Said
    Bibi, Noor Shad
    Muhammad, Noor
    Qasim, Muhammad
    IRANIAN JOURNAL OF CHEMISTRY & CHEMICAL ENGINEERING-INTERNATIONAL ENGLISH EDITION, 2020, 39 (06): : 281 - 292
  • [10] Simultaneous biosynthesis and purification of two extracellular Bacillus hydrolases in aqueous two-phase systems
    Ivanova, V
    Yankov, D
    Kabaivanova, L
    Pashkoulov, D
    MICROBIOLOGICAL RESEARCH, 2001, 156 (01) : 19 - 30
12345