Molecular Pathway of Psoralidin-Induced Apoptosis in HepG2 Cell Line

被引:9
作者
Yu Bin [1 ]
Wang An-hong [2 ,3 ]
Zhou Kun [2 ,4 ]
Chai Li-juan [2 ,4 ]
Liu Lu [5 ]
机构
[1] Tianjin Univ Tradit Chinese Med, Sch Integrat Med, Tianjin 300193, Peoples R China
[2] Tianjin Univ Tradit Chinese Med, Inst Tradit Chinese Med, Tianjin 300193, Peoples R China
[3] Gansu Prov Hosp, Dept Pharm, Lanzhou 731600, Gansu, Peoples R China
[4] Tianjin State Key Lab Modern Chinese Med, Tianjin 300193, Peoples R China
[5] Henan Univ, Coll Pharm, Kaifeng 475004, Henan, Peoples R China
基金
中国国家自然科学基金;
关键词
psoralidin; apoptosis; HepG2; mitochondria; p53; cyclosporin A; CORYLIFOLIA L; PROTEIN; DEATH; PROLIFERATION; CYCLOSPORINE; ACTIVATION; EXPRESSION; CLEAVAGE; RELEASE; BAX;
D O I
10.1007/s11655-016-2251-5
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Objective To test the role of psoralidin in human liver cancer HepG2 cells in vitro. Methods Cell viability was assessed by methylthiazolyldiphenyl-tetrazolum bromide assay and apoptotic cells were labeled by annexin V then sorted by flow cytometry. Protein expressions of caspase-3, caspase-8, caspase-9, Bax, Bid, Bcl-2, Bcl-xL and p53 were examined by western blot while activity of caspase-3, -8 and -9 were also determined. Results Psoralidin reduces cell viability greatly in a time dependent manner (64%, 40%, 21%, 12% at 2, 6, 24 and 48 h treatment with 64 mu mol/L psoralidin respectively) and up-regulates activities of caspase-3, -8 and -9 in a concentration dependent manner (between 4 to 64 mu mol/L). Psoralidin also increases the expression of pro-apoptosis genes Bax, Bid and p53 while decreases the expression of pro-survival genes Bcl-2 and Bcl-xL, both in a concentration dependent manner between 4 and 64 mu mol/L (P<0.05 at 16 and 64 mu mol/L). Caspase-3 inhibitor (Ac-DEVD-CHO at concentrations between 10 to 20 mu mol/L), p53 inhibitor (pifithrin-alpha at 5 mu mol/L) and cyclosporin A can attenuate the apoptotic effect of psoralidin. Conclusion The cytotoxic role of psoralidin might work through both intrinsic and extrinsic apoptotic pathway.
引用
收藏
页码:757 / 762
页数:6
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