Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, Sao Paulo, Brazil

被引：16

作者：

de Melo, Gessica Baptista ^{[1
]}

Malta, Fernanda de Mello ^{[2
]}

Maruta, Celina Wakisaka ^{[3
]}

Criado, Paulo Ricardo ^{[3
,4
]}

Pagliusi Castilho, Vera Lucia ^{[5
]}

do Nascimento Goncalves, Elenice Messias ^{[5
]}

de Carvalho do Espirito-Santo, Maria Cristina ^{[1
]}

de Paula, Fabiana Martins ^{[1
]}

Borges Gryschek, Ronaldo Cesar ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Med, Inst Med Trop Sao Paulo, Lab Imunopatol Esquistossomose LIM 06, Sao Paulo, Brazil

[2] Univ Sao Paulo, Fac Med, Inst Med Trop Sao Paulo, Lab Gastroenterol & Hepatol Trop LIM 07, Sao Paulo, Brazil

[3] Univ Sao Paulo, Hosp Clin, Fac Med, Dept Dermatol, Sao Paulo, Brazil

[4] Fac Med ABC, Sao Paulo, Brazil

[5] Univ Sao Paulo, Hosp Clin, Fac Med, Div Lab Cent, Sao Paulo, Brazil

来源：

PARASITE EPIDEMIOLOGY AND CONTROL | 2019年 / 7卷

基金：

巴西圣保罗研究基金会;

关键词：

Blastocystis sp; Subtype; Urticaria; Brazil;

D O I：

10.1016/j.parepi.2019.e00124

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Blastocystis sp. is described as an enteric protist prevalent in fecal samples from humans and animals; its pathogenicity and epidemiology are still controversial. Currently, it has been associated with intestinal diseases such as irritable bowel syndrome and clinical manifestations of allergic skin, such as chronic urticaria. In the context of urticaria, it is still uncertain whether this organism is directly related to the allergic manifestation or just a common component of the intestinal microbiota. This study aimed to evaluate the occurrence and molecular diversity of Blastocystis sp. in individuals with urticaria from a dermatology outpatient clinic, Sao Paulo, Brazil. Fecal samples of 58 patients with urticaria were examined using parasitological methods; and subsequently tested by polymerase chain reaction using Blastocystis-specific primers. The subtypes (STs) and alleles (a) were determined using BLASTn and MLST tools. ST1, ST2, ST3, ST4, ST6 and mixed infection (ST1 + ST3) were identified in the patients with urticaria; ST1 (a4), ST3 (a34 and a36) and ST4 (a42) were the most prevalent. Our molecular analyses allowed an initial description of Blastocystis subtypes in patients with urticaria from Sao Paulo city, Brazil. (C) 2019 Published by Elsevier Ltd on behalf of World Federation of Parasitologists.

引用

页数：6

共 37 条

[1] Subtype Distribution of Blastocystis Isolates in Sebha, Libya [J].

Abdulsalam, Awatif M. ;

Ithoi, Init ;

Al-Mekhlafi, Hesham M. ;

Al-Mekhlafi, Abdulsalam M. ;

Ahmed, Abdulhamid ;

Surin, Johari .

PLOS ONE, 2013, 8 (12)

[2] Variable geographic distribution of Blastocystis subtypes and its potential implications [J].

Alfellani, Mohammed A. ;

Stensvold, C. Rune ;

Vidal-Lapiedra, Amparo ;

Onuoha, Emeh Sunday Uche ;

Fagbenro-Beyioku, Adetayo F. ;

Clark, C. Graham .

ACTA TROPICA, 2013, 126 (01) :11-18

[3]

Bakiri Alketa Hysni, 2010, J Clin Med Res, V2, P247, DOI 10.4021/jocmr456w

[4] Do not forget the stool examination!-cutaneous and gastrointestinal manifestations of Blastocystis sp infection [J].

Balint, Anita ;

Doczi, Ilona ;

Bereczki, Laszlo ;

Gyulai, Rolland ;

Szucs, Monika ;

Farkas, Klaudia ;

Urban, Edit ;

Nagy, Ferenc ;

Szepes, Zoltan ;

Wittmann, Tibor ;

Molnar, Tamas .

PARASITOLOGY RESEARCH, 2014, 113 (04) :1585-1590

[5] Intestinal parasite infections in a rural community of Rio de Janeiro (Brazil): Prevalence and genetic diversity of Blastocystis subtypes [J].

Barbosa, Carolina Valenca ;

Barreto, Magali Muniz ;

Andrade, Rosemary de Jesus ;

Sodre, Fernando ;

d'Avila-Levy, Claudia Masini ;

Peralta, Jose Mauro ;

Igreja, Ricardo Pereira ;

de Macedo, Heloisa Werneck ;

Carneiro Santos, Helena Lucia .

PLOS ONE, 2018, 13 (03)

[6] Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil [J].

Barbosa, Carolina Valenca ;

Batista, Rosemary de Jesus ;

Igreja, Ricardo Pereira ;

d'Avila Levy, Claudia Masini ;

de Macedo, Heloisa Werneck ;

Carneiro Santos, Helena Lucia .

PARASITES & VECTORS, 2017, 10

[7] Remission of delayed pressure urticaria after eradication of Blastocystis hominis [J].

Cassano, N ;

Scoppio, BM ;

Loviglio, MC ;

Vena, GA .

ACTA DERMATO-VENEREOLOGICA, 2005, 85 (04) :357-358

[8]

Criado P.R., 2011, AN BRAS DERMATOL, V88, P355

[9] Blastocystis and urticaria: Examination of subtypes and morphotypes in an unusual clinical manifestation [J].

Daniel Casero, Rodolfo ;

Mongi, Florencia ;

Sanchez, Angie ;

David Ramirez, Juan .

ACTA TROPICA, 2015, 148 :156-161

[10] Molecular characterization of intestinal protozoa in two poor communities in the State of Sao Paulo, Brazil [J].

David, Erica Boarato ;

Guimaraes, Semiramis ;

de Oliveira, Ana Paula ;

Goulart de Oliveira-Sequeira, Teresa Cristina ;

Bittencourt, Gabriela Nogueira ;

Moraes Nardi, Ana Rita ;

Martins Ribolla, Paulo Eduardo ;

Bueno Franco, Regina Maura ;

Branco, Nilson ;

Tosini, Fabio ;

Bella, Antonino ;

Pozio, Edoardo ;

Caccio, Simone M. .

PARASITES & VECTORS, 2015, 8

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