Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum

被引：135

作者：

Fukuta, S ^{[1
]}

Ohishi, K ^{[1
]}

Yoshida, K ^{[1
]}

Mizukami, Y ^{[1
]}

Ishida, A ^{[1
]}

Kanbe, M ^{[1
]}

机构：

[1] Aichi Prefecture Agr Res Ctr, Nagakute, Aichi 4801193, Japan

来源：

JOURNAL OF VIROLOGICAL METHODS | 2004年 / 121卷 / 01期

关键词：

TSWV; IC/RT-LAMP; detection; chrysanthemum;

D O I：

10.1016/j.jviromet.2004.05.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An immunocapture reverse transcription loop-mediated isothermal amplification (IC/RT-LAMP) was developed for the detection of tomato spotted wilt virus (TSWV) from chrysanthemum. This method enabled sensitive, reproducible and specific detection of TSWV from chrysanthemum plants. In the RT-LAMP method, TSWV genomic RNA could be amplified under isothermal (65degreesC) conditions within 1 h. The resulting amplicons were detected by the measurement or observation of the turbidity of the reaction mixture without gel electrophoresis. IC/RT-LAMP was 100 times more sensitive than IC/RT-PCR. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：49 / 55

页数：7

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