Unique Aggregation of Retroviral Particles Pseudotyped with the Delta Variant SARS-CoV-2 Spike Protein

被引：4

作者：

Petersen, Jennifer D. ^{[1
]}

Lu, Jianming ^{[2
]}

Fitzgerald, Wendy ^{[3
]}

Zhou, Fei ^{[4
]}

Blank, Paul S. ^{[1
]}

Matthies, Doreen ^{[4
]}

Zimmerberg, Joshua ^{[1
]}

机构：

[1] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Integrat Biophys, Div Basic & Translat Biophys, NIH, Bethesda, MD 20892 USA

[2] Codex BioSolut Inc, Dept Res & Dev, Cell Biol, 12358 Parklawn Dr,Suite 250, North Bethesda, MD 20852 USA

[3] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Intercellular Interact, Div Basic & Translat Biophys, NIH, Bethesda, MD 20892 USA

[4] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Unit Struct Biol, Div Basic & Translat Biophys, NIH, Bethesda, MD 20892 USA

来源：

VIRUSES-BASEL | 2022年 / 14卷 / 05期

关键词：

coronavirus; SARS-CoV-2; pseudotyped viral particle; spike protein; variant; aggregation; RESPIRATORY SYNDROME CORONAVIRUS; RECEPTOR-BINDING; CELL ENTRY; 2019-NCOV; DOMAIN; BETA; ACE2;

D O I：

10.3390/v14051024

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Individuals infected with the SARS-CoV-2 Delta variant, lineage B.1.617.2, exhibit faster initial infection with a higher viral load than prior variants, and pseudotyped viral particles bearing the SARS-CoV-2 Delta variant spike protein induce a faster initial infection rate of target cells compared to those bearing other SARS-CoV-2 variant spikes. Here, we show that pseudotyped viral particles bearing the Delta variant spike form unique aggregates, as evidenced by negative stain and cryogenic electron microscopy (EM), flow cytometry, and nanoparticle tracking analysis. Viral particles pseudotyped with other SARS-CoV-2 spike variants do not show aggregation by any of these criteria. The contribution to infection kinetics of the Delta spike's unique property to aggregate is discussed with respect to recent evidence for collective infection by other viruses. Irrespective of this intriguing possibility, spike-dependent aggregation is a new functional parameter of spike-expressing viral particles to evaluate in future spike protein variants.

引用

页数：15

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