Homogeneous Visual and Fluorescence Detection of Circulating Tumor Cells in Clinical Samples via Selective Recognition Reaction and Enzyme-Free Amplification

被引：115

作者：

Chen, Piaopiao ^{[1
,2
]}

Wang, Yue ^{[1
,2
]}

He, Yaqin ^{[1
,2
]}

Huang, Ke ^{[3
]}

Wang, Xiu ^{[3
]}

Zhou, Ronghui ^{[4
]}

Liu, Tangyuheng ^{[1
,2
]}

Qu, Runlian ^{[1
,2
]}

Zhou, Juan ^{[1
,2
]}

Peng, Wu ^{[1
,2
]}

Li, Mei ^{[1
,2
]}

Bai, Yunjin ^{[1
,2
]}

Chen, Jie ^{[1
,2
]}

Huang, Jin ^{[1
,2
]}

Geng, Jia ^{[1
,2
]}

Xie, Yi ^{[1
,2
]}

Hu, Walter ^{[1
,2
]}

Ying, Binwu ^{[1
,2
]}

机构：

[1] Sichuan Univ, West China Hosp, Dept Lab Med, State Key Lab Biotherapy, Chengdu 610041, Sichuan, Peoples R China

[2] Sichuan Univ, Canc Ctr, Med X Ctr Mfg, West China Precis Med Ind Technol Inst,Dept Urol, Chengdu 610041, Sichuan, Peoples R China

[3] Sichuan Normal Univ, Coll Chem & Mat Sci, Chengdu 610068, Sichuan, Peoples R China

[4] Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China

来源：

ACS NANO | 2021年 / 15卷 / 07期

基金：

中国博士后科学基金;

关键词：

lung cancer CTCs; homogeneous; visual and fluorescence; CdTe QDs; enzyme-free amplification; point-of-care testing; clinical whole blood samples;

D O I：

10.1021/acsnano.1c02080

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Here we report a simple all-nucleic-acid enzyme-free catalyzed hairpin assembly assisted amplification strategy with quantum dots (QDs) as the nanoscale signal reporter for homogeneous visual and fluorescent detection of A549 lung cancer cells from clinical blood samples. This work was based on the phenomenon that CdTe QDs can selectively recognize Ag+ and C-Ag+-C and by using mucin 1 as the circulating tumor cells (CTCs) marker and aptamer as the recognition probe. Under optimized conditions, the limits of detections as low as 0.15 fg/mL of mucin 1 and 3 cells/mL of A549 cells were achieved with fluorescence signals. A 1 fg/mL concentration of mucin 1 and 100 cells/mL of A549 can be distinguished by the naked eye. This method was used to quantitatively analyze CTCs in 51 clinical whole blood samples of patients with lung cancer. The levels of CTCs detected in clinical samples by this method were consistent with those obtained using the folate receptor-polymerase chain reaction clinical test kit and correlated with radiologic and pathological findings.

引用

页码：11634 / 11643

页数：10

共 39 条

[1] OPINION Challenges in circulating tumour cell research [J].