Characterization of Yeast Strains with Ketoreductase Activity for Bioreduction of Ketones

被引：4

作者：

Csuka, Pal ^{[1
]}

Nagy-Gyor, Laszlo ^{[1
]}

Molnar, Zsofia ^{[1
,2
]}

Paizs, Csaba ^{[3
]}

Bodai, Viktoria ^{[4
]}

Poppe, Laszlo ^{[1
,3
,5
]}

机构：

[1] Budapest Univ Technol & Econ, Fac Chem Technol & Biotechnol, Dept Organ Chem & Technol, Muegyet Rkp 3, H-1111 Budapest, Hungary

[2] Hungarian Acad Sci HAS, ELKH Res Ctr Nat Sci, Inst Enzymol, Magyar Tudosok Krt 2, H-1117 Budapest, Hungary

[3] Babes Bolyai Univ, Fac Chem & Chem Engn, Biocatalysis & Biotransformat Res Ctr, Arany Janos Str 11, RO-400028 Cluj Napoca, Romania

[4] Fermentia Microbiol Ltd, Berlini Ut 47-49, H-1049 Budapest, Hungary

[5] SynBiocat LLC, Szilasliget U 3, H-1172 Budapest, Hungary

来源：

PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING | 2021年 / 65卷 / 03期

关键词：

ketoreductase; yeast; whole-cell bioreduction; cofactor regeneration; Design of Experiments (DOE); CARBONYL REDUCTASE; AROMATIC KETONES; BUILDING-BLOCKS; BIOCATALYST; REGENERATION; DESIGN;

D O I：

10.3311/PPch.17429

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

This study describes six yeast strains for stereoselective ketone reductions. The reaction conditions for the yeast strains (Pichia carsonii, Lodderomyces elongisporus, Candida norvegica, Candida guillermondi, Debaromyces fabryi and Candida parapsilosis) were optimized in a design of experiments for three ketones of different properties. The pH tolerance, temperature stability and productivity of the bioreductions with lyophilized cells of the yeast strains were characterized. In several cases, the optimized bioreductions resulted in enantiopure alcohols (ee> 99 %) with conversions ranging from moderate to excellent.

引用

页码：299 / 307

页数：9

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