Effects of pH and ionic strength on the rheology and microstructure of a pressure-induced whey protein gel

被引:19
作者
He, Jin-Song [1 ,2 ]
Azuma, Norihiro [1 ]
Yang, Hongwei [2 ]
机构
[1] Utsunomiya Univ, Dept Appl Biochem, Utsunomiya, Tochigi 3218505, Japan
[2] Tsinghua Univ, Dept Environm Sci & Engn, Beijing 100084, Peoples R China
关键词
BETA-LACTOGLOBULIN; PHASE-SEPARATION; OPTIMIZATION; HEAT;
D O I
10.1016/j.idairyj.2009.08.006
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The effects of pH and ionic strength (1) on the properties of a pressure-induced gel from a whey protein isolate (0.2 g mL(-1)) were studied using a series of buffers with different pH and ionic strength (1). The theological properties and water-holding capacity (WHC) of the gel changed in a complex pattern with pH and I but could be successfully modelled using an artificial neural network (ANN) model. The degree of cross-linking of S-S bonds was higher at pH 8.0 than at pH 5.0: consequently, a rigid gel was formed at alkaline pH values. The geometric dimensions of the microstructure decreased with increasing pH and decreasing ionic strength, and the WHC mainly depended on the geometric properties of the microstructure. These results indicate that pH and ionic strength influenced the gel properties by controlling the cross-linking reaction and phase separation that occur during gelation. They also confirmed the good potential application of ANN in studies of gelation. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:89 / 95
页数:7
相关论文
共 28 条
[11]   Pressure-induced denaturation of monomer β-lactoglobulin is partially irreversible:: Comparison of monomer form (highly acidic pH) with dimer form (neutral pH) [J].
Ikeuchi, Y ;
Nakagawa, K ;
Endo, T ;
Suzuki, A ;
Hayashi, T ;
Ito, T .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (08) :4052-4059
[12]   Application of genetic algorithm for optimization of vegetable oil hydrogenation process [J].
Izadifar, M. ;
Jahromi, M. Zolghadri .
JOURNAL OF FOOD ENGINEERING, 2007, 78 (01) :1-8
[13]   POTENTIAL FOOD APPLICATIONS OF HIGH-PRESSURE EFFECTS ON ICE-WATER TRANSITIONS [J].
KALICHEVSKY, MT ;
KNORR, D ;
LILLFORD, PJ .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 1995, 6 (08) :253-259
[14]   Influences of saccharides on the pressure-induced gels from a whey protein isolate [J].
Kanno, C ;
He, JS ;
Ametani, M ;
Azuma, N .
TRENDS IN HIGH PRESSURE BIOSCIENCE AND BIOTECHNOLOGY, PROCEEDINGS, 2002, 19 :481-486
[15]   FINE-STRANDED AND PARTICULATE GELS OF BETA-LACTOGLOBULIN AND WHEY-PROTEIN AT VARYING PH [J].
LANGTON, M ;
HERMANSSON, AM .
FOOD HYDROCOLLOIDS, 1992, 5 (06) :523-539
[16]  
Mulvihill D. M., 1987, Food Technology, V41, P110
[17]   Optimization of oven toasting for improving crispness and other quality attributes of ready to eat potato-soy snack using response surface methodology [J].
Nath, A. ;
Chattopadhyay, P. K. .
JOURNAL OF FOOD ENGINEERING, 2007, 80 (04) :1282-1292
[18]  
Needs E, 2002, FOOD ENGN S, P269
[19]   In situ measurements of pH changes in β-lactoglobulin solutions under high hydrostatic pressure [J].
Orlien, Vibeke ;
Olsen, Karsten ;
Skibsted, Leif H. .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (11) :4422-4428
[20]  
PERRIN D D, 1974, P176