Non-invasive electrochemical immunosensor for sweat cortisol based on L-cys/AuNPs/MXene modified thread electrode

被引:108
作者
Laochai, Thidarut [1 ]
Yukird, Jutiporn [2 ]
Promphet, Nadtinan [2 ]
Qin, Jiaqian [2 ]
Chailapakul, Orawon [1 ,3 ]
Rodthongkum, Nadnudda [2 ,4 ]
机构
[1] Chulalongkorn Univ, Fac Sci, Dept Chem, 254 Phayathai Rd, Bangkok 10330, Thailand
[2] Chulalongkorn Univ, Met & Mat Sci Res Inst, Soi Chula 12,Phayathai Rd, Bangkok 10330, Thailand
[3] Chulalongkorn Univ, Fac Sci, Electrochem & Opt Spect Ctr Excellence EOSCE, Dept Chem, 254 Phayathai Rd, Bangkok 10330, Thailand
[4] Chulalongkorn Univ, Ctr Excellence Respons Wearable Mat, Bangkok 10330, Thailand
关键词
MXene; Gold nanoparticle; Thread-based electrochemical immunosensor; Sweat cortisol; Adrenal gland disorder; ECCRINE SWEAT; BIOSENSOR; ASSAY;
D O I
10.1016/j.bios.2022.114039
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Thread-based electrochemical immunosensor is fabricated for non-invasive detection of cortisol in sweat by immobilization of anti-cortisol on L-cys/AuNPs/MXene modified conductive thread electrode. MXene and AuNPs increase the surface area of conductive thread electrode and facilitate anti-cortisol immobilization leading to enhanced sensor sensitivity. Anti-cortisol is immobilized on L-cys/AuNPs/MXene modified electrode by using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysulfosuccinimide coupling agents. The electrochemical detection of cortisol is based on the decrease of oxidation current towards the antigen-antibody binding interaction owing to blocking of electron transfer process by cortisol. Under the optimal conditions, this immunosensor offers high sensitivity, a wide linearity of 5-180 ng mL(-1) and a detection limit of 0.54 ng mL(-1) with negligible effect from interferences. Furthermore, this immunosensor provides high reproducibility and long-term storage stability (>= 6 weeks). Ultimately, this system is successfully applied for the detection of cortisol in artificial sweat with satisfactory results. Hence, this platform might be suitable to apply as a wearable electrochemical sensor for sweat cortisol by integrating on a wristband.
引用
收藏
页数:10
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