Inhibition of tetracycline on anaerobic digestion of swine wastewater

The inhibition of tetracycline on anaerobic digestion of synthetic swine wastewater was examined with a semicontinuous operation for 103 days at a dosage ranging 2-8 mg/L. COD concentrations, VFA compositions in effluents and methane production were measured. The negative effects of tetracycline on the four individual steps of anaerobic digestion and its toxicity on anaerobic microorganisms were also evaluated. Results showed that continuous addition of 8 mg/L tetracycline in the bioreactor resulted in 73.28% reduction of daily methane production and made anaerobic digestion upset. Besides, methanogenesis was particularly inhibited compared to other three steps and the corresponding enzyme activities decreased by 66%. Furthermore, the polysaccharide contents in EPS increased after exposure to tetracycline, which could inhibit direct connections among microorganism. At last, long-term exposure to tetracycline inhibit anaerobic microbial activities and caused liberation of lactate dehydrogenase. The results would provide novel insights for anaerobic digestion of swine wastewater.