15-Deoxy-Δ12,14-prostaglandin J2 Induces Apoptosis and Upregulates SOCS3 in Human Thyroid Cancer Cells

被引：18

作者：

Trindade-da-Silva, Carlos Antonio ^{[1
,2
,3
]}

Reis, Carolina Fernandes ^{[1
,4
]}

Vecchi, Lara ^{[1
]}

Napimoga, Marcelo Henrique ^{[5
]}

Sperandio, Marcelo ^{[5
]}

Matias Colombo, Bruna Franca ^{[1
]}

Alves, Patricia Terra ^{[1
]}

Ward, Laura Sterian ^{[4
]}

Ueira-Vieira, Carlos ^{[2
]}

Goulart, Luiz Ricardo ^{[1
,6
]}

机构：

[1] Univ Fed Uberlandia, Lab Nanobiotechnol, BR-38400902 Uberlandia, MG, Brazil

[2] Univ Fed Uberlandia, Genet Lab, BR-38400902 Uberlandia, MG, Brazil

[3] Univ Calif Davis, Hammock Lab Pesticide Biotechnol, Davis, CA 95616 USA

[4] Univ Estadual Campinas, Lab Canc Mol Genet, BR-13081970 Campinas, SP, Brazil

[5] Sao Leopoldo Mandic Inst & Res Ctr, Lab Immunol & Mol Biol, BR-13045755 Campinas, SP, Brazil

[6] Univ Calif Davis, Dept Med Microbiol & Immunol, Davis, CA 95616 USA

来源：

PPAR RESEARCH | 2016年 / 2016卷

关键词：

ACTIVATED-RECEPTOR-GAMMA; PPAR-GAMMA; STAT3; IL-6; AGONISTS; LIGANDS; GROWTH; PROLIFERATION; TRANSCRIPTION; INFLAMMATION;

D O I：

10.1155/2016/4106297

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The cyclopentenone prostaglandin 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) is a natural ligand of peroxisome proliferator-activated receptor gamma (PPAR-gamma) and a potential mediator of apoptosis in cancer cells. In the present study, we evaluated the effect of 15d-PGJ(2) in human thyroid papillary carcinoma cells (TPC-1) using different doses of 15d-PGJ(2) (0.6 to 20 mu M) to determine IC50 (9.3 mu M) via the MTT assay. The supernatant culture medium of the TPC-1 cells that was treated either with 15d-PGJ(2) or with vehicle (control) for 24 hours was assessed for IL-6 secretion via CBA assay. RT-qPCR was used to evaluate mRNA expression of IL-6, SOCS1, SOCS3, and STAT3. TPC-1 cells treated with 15d-PGJ(2) decreased the secretion and expression of IL-6 and STAT3, while it increased SOCS1 and SOCS3. Overall, we demonstrated that 15d-PGJ(2) downregulated IL-6 signaling pathway and led TPC-1 cells into apoptosis. In conclusion, 15d-PGJ(2) shows the potential to become a new therapeutic approach for thyroid tumors.

引用

页数：8

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