Genetic assessment of watermelon germplasm using the AFLP technique

被引:26
作者
Che, KP
Liang, CY
Wang, YG
Jin, DM
Wang, B [1 ]
Xu, Y
Kang, GB
Zhang, HY
机构
[1] Chinese Acad Sci, Inst Genet, Beijing, Peoples R China
[2] Natl Engn Res Ctr Vegetables, Beijing, Peoples R China
关键词
AFLP; cluster analysis; genetic diversity; watermelon; germplasm identification;
D O I
10.21273/HORTSCI.38.1.81
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Amplified fragment length polymorphism (AFLP) analyses were used to assess genetic diversity among 30 genotypes of watermelon [Citrullus lanatus (Thunb.) Mansf.] representing a broad genetic base, including breeding lines and commercial germplasm. EightAFLP primer combinations selected from 64 primer combinations were polymorphic. The polymorphism was 13.0% to 31.9% within the 28 cultivars examined, and 45.3% to 64.2% among all the genotypes. Each genotype could be successfully distinguished based on AFLP scoring. Cluster grouping of accessions based on the AFLP analysis was consistent with that from classification by pedigrees and ecotypes.
引用
收藏
页码:81 / 84
页数:4
相关论文
共 28 条
[11]  
LI CY, 2000, J BIOTECH SINICA, V16, P91
[12]   Identification of molecular markers in soybean comparing RFLP, RAPD and AFLP DNA mapping techniques [J].
Lin, JJ ;
Kuo, J ;
Ma, J ;
Saunders, JA ;
Beard, HS ;
MacDonald, MH ;
Kenworthy, W ;
Ude, GN ;
Matthews, BF .
PLANT MOLECULAR BIOLOGY REPORTER, 1996, 14 (02) :156-169
[13]   RESISTANCE TO RACES 0, 1, AND 2 OF FUSARIUM-WILT OF WATERMELON IN CITRULLUS-SP PI-296341-FR [J].
MARTYN, RD ;
NETZER, D .
HORTSCIENCE, 1991, 26 (04) :429-432
[14]   RAPID ISOLATION OF HIGH MOLECULAR-WEIGHT PLANT DNA [J].
MURRAY, MG ;
THOMPSON, WF .
NUCLEIC ACIDS RESEARCH, 1980, 8 (19) :4321-4325
[15]   ISOZYME AND SEED PROTEIN PHYLOGENY OF THE GENUS CITRULLUS (CUCURBITACEAE) [J].
NAVOT, N ;
ZAMIR, D .
PLANT SYSTEMATICS AND EVOLUTION, 1987, 156 (1-2) :61-67
[16]   MATHEMATICAL-MODEL FOR STUDYING GENETIC-VARIATION IN TERMS OF RESTRICTION ENDONUCLEASES [J].
NEI, M ;
LI, WH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (10) :5269-5273
[17]  
OYULU C, 1977, TROP SCI, V19, P55
[18]   Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs, and AFLPs [J].
Pejic, I ;
Ajmone-Marsan, P ;
Morgante, M ;
Kozumplick, V ;
Castiglioni, P ;
Taramino, G ;
Motto, M .
THEORETICAL AND APPLIED GENETICS, 1998, 97 (08) :1248-1255
[20]   Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs [J].
Russell, JR ;
Fuller, JD ;
Macaulay, M ;
Hatz, BG ;
Jahoor, A ;
Powell, W ;
Waugh, R .
THEORETICAL AND APPLIED GENETICS, 1997, 95 (04) :714-722