17β-estradiol stimulates flow-induced vasodilatation in isolated small mesenteric arteries from prepubertal female rats

被引:13
作者
Cockell, AP [1 ]
Poston, L [1 ]
机构
[1] United Med & Dent Sch Guys & St Thomas Hosp, Div Obstet & Gynaecol, Fetal Hlth Res Grp, London SE1 7EH, England
关键词
17; beta-estradiol; small artery; nitric oxide; shear stress; blood flow;
D O I
10.1016/S0002-9378(97)70087-5
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: Flow-induced vasodilatation may contribute to lowering of peripheral resistance to pregnancy. This study investigated modulation of flow responses by 17 beta-estradiol. STUDY DESIGN: Small mesenteric arteries from prepubertal female Wistar rats were pretreated for 3 hours with 17 beta-estradiol (10(-7) mol/L) with 17 alpha-estradiol (10(-7) mol/L) or vehicle (0.1% dimethylsulfoxide). Responses to intraluminal flow were evaluated with use of a pressure arteriograph. RESULTS: After pretreatment with 17 beta-estradiol arteries relaxed to flow, whereas those treated with vehicle or 17 alpha-estradiol did not (percent change in diameter at maximum flow rate after 17 beta-estradiol 38.7% +/- 5.7%, n = 10, vs 1.1% +/- 4.3%, n = 10 after vehicle; p < 0.01). Endothelium removal or pretreatment with either a nitric oxide synthase inhibitor or a novel soluble guanylyl cyclase inhibitor diminished the response to 17 beta-estradiol. CONCLUSION: 17 beta-Estradiol stimulated nitric oxide-mediated flow-induced relaxation. Through this pathway 17 beta-estradiol could play an important role in the control of vascular tone.
引用
收藏
页码:1432 / 1438
页数:7
相关论文
共 25 条
[1]   Ethinylestradiol does not enhance the expression of nitric oxide synthase in bovine endothelial cells but increases the release of bioactive nitric oxide by inhibiting superoxide anion production [J].
Arnal, JF ;
Clamens, S ;
Pechet, C ;
NegreSalvayre, A ;
Allera, C ;
Girolami, JP ;
Salvayre, R ;
Bayard, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) :4108-4113
[2]   ESTROGEN-INDUCED SENSITIZATION OF UTERINE ARTERY OF GUINEA-PIG TO ACETYLCHOLINE [J].
BELL, C .
BRITISH JOURNAL OF PHARMACOLOGY, 1973, 49 (04) :595-601
[3]   ESTROGEN PRETREATMENT DIRECTLY POTENTIATES ENDOTHELIUM-DEPENDENT VASORELAXATION OF PORCINE CORONARY-ARTERIES [J].
BELL, DR ;
RENSBERGER, HJ ;
KORITNIK, DR ;
KOSHY, A .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1995, 268 (01) :H377-H383
[4]   NITRIC-OXIDE DIRECTLY ACTIVATES CALCIUM-DEPENDENT POTASSIUM CHANNELS IN VASCULAR SMOOTH-MUSCLE [J].
BOLOTINA, VM ;
NAJIBI, S ;
PALACINO, JJ ;
PAGANO, PJ ;
COHEN, RA .
NATURE, 1994, 368 (6474) :850-853
[5]  
Cheng David Y., 1994, Life Sciences, V55, P187
[6]   Isolated mesenteric arteries from pregnant rats show enhanced flow-mediated relaxation but normal myogenic tone [J].
Cockell, AP ;
Poston, L .
JOURNAL OF PHYSIOLOGY-LONDON, 1996, 495 (02) :545-551
[7]   Flow-mediated vasodilatation is enhanced in normal pregnancy but reduced in preeclampsia [J].
Cockell, AP ;
Poston, L .
HYPERTENSION, 1997, 30 (02) :247-251
[8]   FLOW ACTIVATES AN ENDOTHELIAL POTASSIUM CHANNEL TO RELEASE AN ENDOGENOUS NITROVASODILATOR [J].
COOKE, JP ;
ROSSITCH, E ;
ANDON, NA ;
LOSCALZO, J ;
DZAU, VJ .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (05) :1663-1671
[9]   FLOW-MEDIATED ENDOTHELIAL MECHANOTRANSDUCTION [J].
DAVIES, PF .
PHYSIOLOGICAL REVIEWS, 1995, 75 (03) :519-560
[10]  
GISCLARD V, 1988, J PHARMACOL EXP THER, V244, P16