Multilayer membranes via layer-by-layer deposition of glucose oxidase and Au nanoparticles on a Pt electrode for glucose sensing

被引：20

作者：

Hoshi, Tomonori ^{[1
]}

Sagae, Naoki ^{[1
]}

Daikuhara, Kenji ^{[1
]}

Takahara, Kei ^{[1
]}

Anzai, Jun-ichi ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Pharmaceut Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2007年 / 27卷 / 04期

关键词：

layer-by-layer deposition; gold nanoparticle; glucose oxidase; glucose sensor;

D O I：

10.1016/j.msec.2006.10.004

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We prepared multilayer membranes by the layer-by-layer deposition of glucose oxidase (GOx) and Au nanoparticles (5, 10, or 50 nm 9) on sensor substrates, such as a Pt electrode and a quartz glass plate, to prepare glucose sensors. The enzyme activity of GOx remained even in alternate assemblies, and the activity increased with the increasing number of depositions. The apparent K-m values of the deposited GOx were 28-32 mM, while a reported value in a solution is 33 mM. These results suggest that Au nanoparticles can be used as binders for the deposition of GOx without significant change in the affinity between GOx and glucose. (C) 2006 Elsevier B.V. All rights reserved.

引用

页码：890 / 894

页数：5

共 15 条

[1] Layer-by-layer construction of enzyme multilayers on an electrode for the preparation of glucose and lactate sensors: Elimination of ascorbate interference by means of an ascorbate oxidase multilayer [J].

Anzai, J ;

Takeshita, H ;

Kobayashi, Y ;

Osa, T ;

Hoshi, T .

ANALYTICAL CHEMISTRY, 1998, 70 (04) :811-817

[2] A layer-by-layer deposition of concanavalin A and native glucose oxidase to form multilayer thin films for biosensor applications [J].

Anzai, J ;

Kobayashi, Y ;

Hoshi, T ;

Saiki, H .

CHEMISTRY LETTERS, 1999, (04) :365-366

[3] Use of Con A and mannose-labeled enzymes for the preparation of enzyme films for biosensors [J].

Anzai, J ;

Kobayashi, Y ;

Nakamura, N ;

Hoshi, T .

SENSORS AND ACTUATORS B-CHEMICAL, 2000, 65 (1-3) :94-96

[4] Refined structure of an intact IgG2a monoclonal antibody [J].

Harris, LJ ;

Larson, SB ;

Hasel, KW ;

McPherson, A .

BIOCHEMISTRY, 1997, 36 (07) :1581-1597

[5] CRYSTAL-STRUCTURE OF GLUCOSE-OXIDASE FROM ASPERGILLUS-NIGER REFINED AT 2 .3 ANGSTROM RESOLUTION [J].

HECHT, HJ ;

KALISZ, HM ;

HENDLE, J ;

SCHMID, RD ;

SCHOMBURG, D .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 229 (01) :153-172

[6] CONTROLLED DEPOSITION OF GLUCOSE-OXIDASE ON PLATINUM-ELECTRODE BASED ON AN AVIDIN-BIOTIN SYSTEM FOR THE REGULATION OF OUTPUT CURRENT OF GLUCOSE SENSORS [J].

HOSHI, T ;

ANZAI, J ;

OSA, T .

ANALYTICAL CHEMISTRY, 1995, 67 (04) :770-774

[7]

HOSHI T, 2005, ITE LETT, V6, P539

[8]

HOSHI T, 2005, ITE LETT, V6, P322

[9] Multilayer membranes via layer-by-layer deposition of ascorbate oxidase and Au nanoparticles on the Pt electrode for reduction of oxidation current derived from ascorbate [J].

Hoshi, Tomonori ;

Sagae, Naoki ;

Daikuhara, Kenji ;

Anzai, Jun-ichi .

TALANTA, 2007, 71 (02) :644-647

[10] Activity and stability of glucose oxidase in molecular films assembled alternately with polyions [J].

Onda, M ;

Ariga, K ;

Kunitake, T .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 87 (01) :69-75

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