Cross-Sectional Evaluation of Humoral Responses against SARS-CoV-2 Spike

被引：162

作者：

Prevost, Jeremie ^{[1
,2
]}

Gasser, Romain ^{[1
,2
]}

Beaudoin-Bussieres, Guillaume ^{[1
,2
]}

Richard, Jonathan ^{[1
,2
]}

Duerr, Ralf ^{[3
]}

Laumaea, Annemarie ^{[1
,2
]}

Anand, Sai Priya ^{[1
,4
]}

Goyette, Guillaume ^{[1
]}

Benlarbi, Mehdi ^{[1
]}

Ding, Shilei ^{[1
]}

Medjahed, Halima ^{[1
]}

Lewin, Antoine ^{[5
]}

Perreault, Josee ^{[5
]}

Tremblay, Tony ^{[5
]}

Gendron-Lepage, Gabrielle ^{[1
]}

Gauthier, Nicolas ^{[6
]}

Carrier, Marc ^{[7
]}

Marcoux, Diane ^{[8
]}

Piche, Alain ^{[9
]}

Lavoie, Myriam ^{[10
]}

Benoit, Alexandre ^{[11
]}

Loungnarath, Vilayvong ^{[12
]}

Brochu, Gino ^{[13
,14
]}

Haddad, Elie ^{[2
,16
,20
]}

Stacey, Hannah D. ^{[21
]}

Miller, Matthew S. ^{[21
]}

Desforges, Marc ^{[15
,16
]}

Talbot, Pierre J. ^{[15
]}

Maule, Graham T. Gould ^{[17
,18
]}

Cote, Marceline ^{[17
,18
]}

Therrien, Christian ^{[19
]}

Serhir, Bouchra ^{[19
]}

Bazin, Renee ^{[5
]}

Roger, Michel ^{[1
,2
,19
]}

Finzi, Andres ^{[1
,2
,4
]}

机构：

[1] CHUM, Ctr Rech, Montreal, PQ H2X 0A9, Canada

[2] Univ Montreal, Dept Microbiol Infectiol & Immunol, Montreal, PQ H2X 0A9, Canada

[3] NYU, Sch Med, Dept Pathol, New York, NY 10016 USA

[4] McGill Univ, Dept Microbiol & Immunol, Montreal, PQ H3A 2B4, Canada

[5] Hema Quebec, Affaires Med & Innovat, Quebec City, PQ G1V 5C3, Canada

[6] Hop Sacre Coeur Montreal, Montreal, PQ H4J 1C5, Canada

[7] Hop Cite de la Sante, Laval, PQ H7M 3L9, Canada

[8] Hotel Dieu Levis, Levis, PQ G6V 3Z1, Canada

[9] CHU Sherbrooke, Sherbrooke, PQ J1H 5H4, Canada

[10] Hop Chicoutimi, CIUSSS Saguenay Lac St Jean, Chicoutimi, PQ G7H 5H6, Canada

[11] Hop Verdun, Montreal, PQ H4G 2A3, Canada

[12] CHU Quebec, Hop Enfant Jesus, Quebec City, PQ G1J 1Z4, Canada

[13] CIUSSS Mauricie, Trois Rivieres, PQ G9A 5C5, Canada

[14] Ctr Quebec, Trois Rivieres, PQ G9A 5C5, Canada

[15] INRS Inst Armand Frappier, Laval, PQ H7V 1B7, Canada

[16] CHU Ste Justine, Montreal, PQ H3T 1C5, Canada

[17] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada

[18] Univ Ottawa, Ctr Infect Immun & Inflammat, Ottawa, ON K1H 8M5, Canada

[19] Inst Natl Sante Publ Quebec, Lab Sante Publ Quebec, Ste Anne De Bellevue, PQ H9X 3R5, Canada

[20] Univ Montreal, Dept Pediat, Montreal, PQ H3T 1C5, Canada

[21] McMaster Univ, Master Immunol Res Ctr, Micheal G DeGroote Inst Infect Dis Res, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada

来源：

CELL REPORTS MEDICINE | 2020年 / 1卷 / 07期

基金：

加拿大健康研究院;

关键词：

SARS CORONAVIRUS; PROTEIN;

D O I：

10.1016/j.xcrm.2020.100126

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

SARS-CoV-2 is responsible for the coronavirus disease 2019 (COVID-19) pandemic, infecting millions of people and causing hundreds of thousands of deaths. The Spike glycoproteins of SARS-CoV-2 mediate viral entry and are the main targets for neutralizing antibodies. Understanding the antibody response directed against SARS-CoV-2 is crucial for the development of vaccine, therapeutic, and public health interventions. Here, we perform a cross-sectional study on 106 SARS-CoV-2-infected individuals to evaluate humoral responses against SARS-CoV-2 Spike. Most infected individuals elicit anti-Spike antibodies within 2 weeks of the onset of symptoms. The levels of receptor binding domain (RBD)-specific immunoglobulin G (IgG) persist over time, and the levels of anti-RBD IgM decrease after symptom resolution. Although most individuals develop neutralizing antibodies within 2 weeks of infection, the level of neutralizing activity is significantly decreased over time. Our results highlight the importance of studying the persistence of neutralizing activity upon natural SARS-CoV-2 infection.

引用

页数：12

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