Quantitative phase imaging of cells through turbid media based on infrared digital holographic microscopy

被引：6

作者：

Lu, Junsheng ^{[1
,2
]}

Zeng, Yanan ^{[3
]}

Chang, Xinyu ^{[1
,2
]}

Hong, Yujian ^{[1
,2
]}

Hu, Xiaodong ^{[1
,2
]}

机构：

[1] Tianjin Univ, State Key Lab Precis Measuring Technol & Instrume, Tianjin 300072, Peoples R China

[2] Tianjin Univ, Nanchang Inst Microtechnol, Tianjin 300072, Peoples R China

[3] Tianjin Agr Univ, Coll Engn & Technol, Tianjin 300000, Peoples R China

来源：

APPLIED PHYSICS EXPRESS | 2021年 / 14卷 / 03期

关键词：

quantitative phase microscopy; turbid media; near-infrared; digital holographic microscopy; optical imaging; MICROFLUIDIC CHANNELS; CONJUGATION; BEAMS;

D O I：

10.35848/1882-0786/abe601

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Most biological cells live in turbid media that poses an obstacle to real-time measurement of morphology, which is significant in the biomedical field. Imaging through turbid environments is experimentally challenging using visible-light illumination for multiple scattering. Therefore, an infrared digital holographic method is reported herein to test biological samples through turbid media that can achieve real-time three-dimensional morphology measurement. Pure milk is employed as turbid media with 500 mu m maximum thickness. The correctness of phase reconstructed from the infrared system is proved compared to that of a visible-light system with less than a 10% deviation of phase difference.

引用

页数：6

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